Description | Z-VAD-FMKisacell-permeable,pan-caspaseinhibitor. |
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IC50&Target | pan-caspase[1] |
InVitro | Z-VAD-FMKisabroad-spectrumcaspaseinhibitor,hasbeenshowntoinhibittheintracellularactivationofcaspase-likeproteases.TheinjectionofZ-VAD-FMKsuppressesthecaspase-3activityinlungtissues,andsignificantlydecreasesthenumberofterminaldUTPnick-endlabeling-positivecells[1].Z-VAD-FMKisadmiNISTeredintraperitoneallyat1hourbeforeand6hoursafterSAH.Expressionofcaspase-3andpositiveTUNELisexaminedasMarkersforapoptosis.Z-VAD-FMKsuppressesTUNELandcaspase-3staininginendothelialcells,decreasescaspase-3activation,reducesBBBpermeABIlity,relievesvasospasm,abolishesbrainedema,andimprovesneurologicaloutcome[2].Z-VAD-FMKisacell-permeablecaspaseinhibitor,efficientlyblockscelldeathinducedbySMNdeficiency[3]. |
InVivo | ThesurvivalrateofmiceisprolongedsignificantlybytheinjectionofZ-VAD-FMK.AllmicesuccumbedtoLPSwithin30hours.Bycontrast,themicetreatedwithZ-VAD-FMKsurvivesignificantlylongerand27%ofthemicesurvivedmorethan7days[1]. |
References |
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PreparingStockSolutions |
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent. | ||||||||||||||||
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CellAssay [3] | Z-VAD-FMKisdissolvedinDMSOandstored,andthendilutedwithappropriatemediumbeforeuse[3]. PCRproductscontainingcodingsequencesforthedSMN(forwardprimer:5′-TAATACGACTCACTATAGGGAAGACGTACGACGAGTCG-3′;andreverseprimer:5′-TAATACGACTCACTATAGGGGTGGTGCTGGCTTCTTTC-3′;productlength,601bps;boldanditalicslettersrepresentT7promotersequences)andcontrolDrosophilaPresenilin(dPsn)gene(forwardprimer:5′-TAATACGACTCACTATAGGGTGGCTGCTGTCAATCTC-3′;andreverseprimer:5′-TAATACGACTCACTATAGGGCGATAGCAACGCTTCTTG-3′;productlength:543bps)areobtainedandgel-purified.Double-strandedRNAs(dsRNA)aregeneratedbytranscriptionwithRibomaxT7TranscriptionkitanddigestedwithRnase-freeDNase.ThedsRNAproductsareethanolprecipitatedandannealedbyincubationat65°Cfor30minandthenslowlyallowedtocoolatroomtemperature.TheannealeddsRNAproductsareanalyzedona1%agaorsegeltoensurethemajorityofdsRNAexistedasasingleband.ThedsRNA(2μg)and/orplasmidDNAs(2μg)areintroducedintocellsbyusingCellfectin.Caspaseinhibitionisachievedbyusing50μMofZ-VAD-FMKintheculturemedium[3].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly. | ||||||||||||||||
AnimalAdministration [1][2] | Z-VAD-FMKisdissolvedat2mg/mLin1%DMSOinsterilesaline(Mice)[1]. Mice[1] | ||||||||||||||||
References |
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MolecularWeight | 467.49 | ||||||||||||
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Formula | C₂₂H₃₀FN₃O₇ | ||||||||||||
CASNo. | 187389-52-2 | ||||||||||||
Storage |
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Shipping | RoomtemperatureincontinentalUS;mayvaryelsewhere | ||||||||||||
Solvent&Solubility | DMSO:≥30mg/mL *"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">1> Purity:>98.0%
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