Description | 5-BrdUisanucleosideanalog,andcompeteswiththymidineforincorporationintoDNA,andisusedinthedetectionofproliferatingcells. |
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InVitro | Bromodeoxyuridineinducesaprogressive,dose-responsivesuppressionofcancercelllineandcancerstemcellpopulationexpansionRG2ratgliomacells.InH9cellsandBJfibroblasts,bromodeoxyuridinealtersthecellcycleprofile[1]. BrdUisstablyintegratedintotheDNA,andthuscanbeusedinassessmentofcellproliferationandothercellprocession[2]. |
InVivo | Bromodeoxyuridine(300mg/kg,i.p.or0.8mg/mL,p.o.)significantlyslowstumorprogressioninratgliomaRG2tumormodel[1]. |
ClinicalTrial | ViewMoreCollapse |
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CellAssay [1] | Culturesareinitiallyplatedat2000cells/cm2 andarequantifiedwithaZ2CoulterCounter.RG2ratgliomacellsaretreatedoncewith0,1,10,or50µMBrdUfor24hours,andcumulativegrowthcurvesareobtainedover18days.Controlandtreatedcellsarequantifiedandreplatedatequaldensitiesondays5,12,and18aftertreatment.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly. References |
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