Deprecated: Creation of dynamic property cls_session::$session_data_table is deprecated in /www/sites/www.188bio.com/index/systems/cls_session.php on line 49
Medchemexpress/Liproxstatin-1/HY-12726/5mg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线
产品资料

Medchemexpress/Liproxstatin-1/HY-12726/5mg

Liproxstatin-1isapotentferroptosisinhibitor,withIC50ofappr38nM.
Description

Liproxstatin-1isapotentferroptosisinhibitor,withIC50ofappr38nM.

IC50&Target

EC50:38nM(ferroptosis)[2]

InVitro

Liproxstatin-1preventsBODIPY581/591C11oxidationinGpx4−/−cells.Moreover,Liproxstatin-1doesnotinterferewithotherclassicaltypesofcelldeath,suchasTNFα-inducedapoptosisandH2O2-inducednecrosis,andinthebonafideL929modelofTNFα/zvad-inducednecroptosis[1].Liproxstatin-1hasgreatantiferroptoticactivitywithEC50ofappr38nM.Fer-1andLiproxstatin-1areinherentlygood,butnotgreat,rADIcal-trappingantioxidants,buttheyareexcellentinphospholipidbilayers.Fer-1(10μM)andLiproxstatin-1(10μM)donotexhibitsignificantinhibitoryactivityinthe15-LOX-1overexpressingcells,andtheconcentrationisalmost1000-foldhigherthantheirEC50sforsubvertingRSL3-inducedferroptosisinthesecells(15and27nM,respectively)[2].

InVivo

Liproxstatin-1(10mg/kg,i.p.)suppressesferroptosisinhumancells,Gpx4−/−kidneyandinanischaemia/reperfusion-inducedtissueinjurymodel[1].

References
  • [1].FriedmannAngeliJP,etal.InactivationoftheferroptosisregulatorGpx4triggersacuterenalfailureinmice.NatCellBiol.2014Dec;16(12):1180-91.

    [2].ZilkaO,etal.OntheMechanismofCytoprotectionbyFerrostatin-1andLiproxstatin-1andtheRoleofLipidPeroxidationinFerroptoticCellDeath.ACSCentSci.2017Mar22;3(3):232-243.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM2.9338mL14.6692mL29.3384mL
5mM0.5868mL2.9338mL5.8677mL
10mM0.2934mL1.4669mL2.9338mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
CellAssay
[1]

ToinducetheknockoutofGpx4,cellsareseededonto96-wellplates(1,000cellsperwell)andtreatedwith1μM4-OH-tamoxifen(TAM)afterplating.CellviABIlityisassessedatdifferenttimepointsaftertreatment(usually72h)usingAquaBluer,unlessstatedotherwise,asanindicatorofviablecells.Alternatively,celldeathisalsoquantifiedbymeasuringreleasedlactatedehydrogenase(LDH)activityusingtheCytotoxicityDetectionKit.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

AnimalAdmiNISTration
[1]

Liproxstatin-1isformulatedin1%dimethylsulphoxide(DMSO)inPBS.

AnimalsincludesinthetreatmentstudyofinducIBLeGpx4−/−miceareequallydistributedbetweensexandweight,withtypically8-10weeksofage.Theaverageweightwithinthegroupsisbetween22and24g.Groupsareformedtohavecomparablenumbersoffemales/malesofthesameage.Animalweightisarrangedtohaveasimilardistributionbetweenfemalesandmales.Forthepharmacologicalinhibitorexperiments,CreERT2;Gpx4fl/flmiceareinjectedonday1and3with0.5mgTAMdissolvedinMiglyol.Onday4,compoundtreatmentisstarted(Liproxstatin-1:10mg/kg)alongwithvehiclecontrol(1%dimethylsulphoxide(DMSO)inPBS).Liproxstatin-1andvehiclecontrolareadministeredoncedailybyi.p.injection.SurvivalanalysisisperformedusingtheGraphPadPrismsoftwareandstatisticalanalysisisdoneaccordingtothelog-ranktest.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References
  • [1].FriedmannAngeliJP,etal.InactivationoftheferroptosisregulatorGpx4triggersacuterenalfailureinmice.NatCellBiol.2014Dec;16(12):1180-91.

    [2].ZilkaO,etal.OntheMechanismofCytoprotectionbyFerrostatin-1andLiproxstatin-1andtheRoleofLipidPeroxidationinFerroptoticCellDeath.ACSCentSci.2017Mar22;3(3):232-243.

MolecularWeight

340.85

Formula

C₁₉H₂₁ClN₄

CASNo.

950455-15-9

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:≥31mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:98.38%