Venor®GeMAdvancecontainsPCRreactiontubespre-coatedwithallPCRreagentstoreducethetotalassaytimewithoutneedtopreparealiquotsofamastermix.ForadditionalconveniencethegelloADIngbufferanddyearealreadyincludedinthereactionbuffer.AfterthermalcyclingthePCRcanbeloadeddirectlyontheagarosegel.
Forconventional,endpointPCR
Applicableinresearchfordirecttestingofcellculturesandcellculture-derivedBIOLOGicals.Intendedforresearchuseonly.Notrecommendedforclinicaldiagnosticsortestingofhumansamples.
Primersets,nucleotides,polymeraseandinternalcontrolDNA,lyophilized,filledin0.2mlPCRreactiontubes
Rehydrationbufferincludinggelloadingbufferandrunningdye
Positivecontrols,lyophilized,filledin0.2mlPCRreactiontubes
PCRgradewater
GelelectrophoresisatendpointofPCR
RequiredConsumables
AgaroseandDNA-specificdyeforgelstaining
Regularblock-heatedPCRcycler
Agarosegelelectrophoresisandgeldocumentationsystem
Pipettingequipment
Tubecentrifuge
Kitcomponentsaremaintainableat+2to+8°Cforatleast6months.AfterrehydratisationoftheindividualPCRtubesthereagentsmustbeusedwithin1hour.
No
Fig.AmplifiedPCRproductsarevisualizedbystandardgelelectrophoresis.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn