ValidationtoolsforconventionalPCRcycler
ISO17025,EN45001,ISO13485,ISO/TS20836:2007,GLP,GMP,IQ/OQ/PQ,andothers.
ThePCRCyclerCheckKitsareavailableforbothconventionalendpointPCRandreal-timePCR(qPCR)cyclers.
FalsenegativePCRresultsarehighlycriticalandmightbecausedbyadefectPCRcycler.Verificationofthecorrecttemperaturecontroloftheusedequipmentisusuallyastressfulissue.TocomplywithqualitymanagementsystemsisnoteasytofulfillforPCRcyclers.
ThePCRCyclerCheckKitsprovidetemperaturesensitivePCRreactionstomonitoranupperandlowertemperaturerangeinonerun.Theprimer/probesequencesinthePCRCyclerCheckKitsweredesignedtoreactextremelysensitivetoincorrecttemperaturecontrol,temperaturehomogeneity,precisionandtiming.Amplificationwillcompletelydropoutattemperaturedifferencesofmorethan2°C.ThecyclerperformanceistestedatstandardPCRsettingstoreflectmostuser’sapplications.Inaddition,thepre-adjustedtargetconcentrationsareonlyamplifiedathighPCRefficienciesasanadditionalindicatorforaccuratetemperaturecontrolofthethermalcycler.
•Noextraequipment,instrumentorsoftwareneeded.
•ApplicablewithanyblockPCRcyclerorqPCRcyclerusedinaresearchorindustrialqualityassurancelabforthereliABIlitytestingofinstrumentperformanceandreliability.
•IntheqPCRThermalCyclerValidiationKittheprobesofeachamplificationsystemarelabeledwithdifferentfluorescentdyes(FAM™andROX™)toallowindividualevaluationofeachtemperaturerange.
•Includesallyouneed:Freeze-driedprimer/probesets,polymerase,nucleotidesandrehydrationbuffer
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn