OurPCRQuantificationStandardscontaingenomicDNAextractedfromlowpassageofdefinedmicroorganisms.TheDNAstartingmaterialwasanalyzedwithstandardizedmethodstodeterminetheDNA’sintegrityandquantity.OnlypureandintactDNAshowinga>10kbbandinanagarosegelandanOD260/280>1.8isused.TheDNAwaspartiallysequencedtoconfirmthespecies’identity.
PCRQuantificationStandardscontainadefinednumberofgenomecopiesofaparticularmicroorganism.Therefore,ourstandardsareperfectlysuitableforgeneratingDNAstandardcurvesinqPCR.Inaddition,standardsarerequiredfortestingthespecificityandsensitivityofPCRassays,particularly,inthecontextofqualitycontrolandincorrespondencewithEP2.6.7.compliance
1vialwith1×108genomes(freeze-dried.)
3vialswithTrisbuffer(10mM,pH8.5)
Thekitmustbestoredat2-8°C(Shippedatambienttemperatures).
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn