MeatID™Screenisakitforthefastandreliableidentificationofseveralanimalspecies(Goat,Sheep,Pork,Horse,Beef,Donkey,Fish,Chicken,TurkeyandCamel)inmeatbyqPCRinasingleparallelrun.
Theidentificationofdifferentmeats,especiallyinmincedmeatproductsisaserioustaskinfoodsafetyandfromandethicalperspective,especiallyforMuslims.Authenticationofforbiddenornonedeclaredingredientssuchasporkorsubstandardmeatisessentialtoensureconfidenceinthesupplychainandregulatorycompliance.
MeatID™Screenisavailableforrapidandreliableanalysisfromvariousmatricesincludingraw,orevenhighlyprocessedandcookedmeatproductswheretheDNAmaybesignificantlydegraded.ItispossIBLetoidentifyrelevantspeciesdowntoathresholdlevelof0.5%withasemi-quantitativeresult.
TaqMan®AssaybasedonFAM™andHEX™labeledprobes.
Canbeusedinresearchandindustryfordetectionofanimalspecies(Goat,Sheep,Pork,Horse,Beef,Donkey,Fish,Chicken,Turkey,Camel,Zebra)inmeatandotherfoodsbyqPCR.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.
Targetsequenceisamitochondrialgene(cytochromeb),whichisavailableinmultiplecopiespercell,enablingsuccessfuldetectionofminuteamountsoftargetDNA!
cyclerbased,real-timePCR
Downtoathresholdlevelof0.5%withasemi-quantitativeresult.
Primersets,nucleotides,polymeraseandinternalcontrolDNA,lyophilized,filledin0.2mlPCRreactiontubes(Stripes).Rehydrationbuffer,Positivecontrol,PCRgradewater.
DNAcanbeextractedfromsamplematerialusingDNAextractionkitse.g.ExtractNow™DNAMiniKit(Art.-Nr.608-1050)orusermethod.
Pipettetips,1.5mlreactiontubes
qPCRcyclerwithFAM™andHEX™filter,benchtopcentrifuge,racksfortubes,variablemicroliterpipettes,sterilefiltertips
approx.90minutes
Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationreagentsmustbestoredat-18°C.
Fig.1:SensitivityoftheMeatID™Screen,parameterHorse.
Fig.2:Detectionof1%w/whorsemeatinrespectivemeatbackground
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn