FoodControl™qPCRisaPCR-kitforfastandreliabledetectionoffoodbornepathogensviareal-timePCR.
DetectingbacterialcontaminationisakeyaspectinfoodmicroBIOLOGy.Consequently,microbiologicalqualitycontrolprogramsareintensifiedthroughoutfoodchainproductioninordertominimizetheriskofintoxicatingconsumers.ReliABIlity,speedandrobustnessofatestsystemtodetectthepresenceorabsence,oreventhedegreeofcontaminationwithpathogens,becomesincreasinglyimportant.FoodControl™qPCRisadiagnosticsystemforeasydeterminationofcontaminationdegreeinagriculturalorfoodindustryviareal-timePCR.
TaqMan®AssaybasedonFAM™andHEX™labeledprobes.
Canbeusedinresearchandindustryfordetectionofpathogeniccontaminationinfoodoragriculturalproducts.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.
Thekitisavailableforthedetectionofthefollowingmicroorganismsviasemi-quantitaivereal-timeorconventionalPCR:
Micro-organism | Target |
Salmonellaenterica | invasionproteinA(invA)gen |
Yersiniaenterocolitica | heatstableenterotoxinAgen |
Shigellaspp. | invasionplasmidantigen(ipaH6)gen |
Campylobacterspp. | acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamineO-acyltransferase(lpxA)gen |
Clostridiumperfringens | phospholipaseCalphatoxin(plc)gen |
Shigelladysenteriae, Escherichiacoli(EHEC) | ShigaToxin1(stx1)gen |
Shigelladysenteriae, Escherichiacoli(EHEC) | ShigaToxin2(stx2)gen |
EscherichiacoliO157 | wBDRgen |
EscherichiacoliO104 | wckDgen |
Listeriaspp. | invasionassociatedproteinp60(iap)gen |
Listeriamonocytogenes | listeriolysinO(hly)gen |
Salmonellaspp. | spacer-regionbetween16Sand23SRNAgenes |
Packagesize25reactionseach:
Cyclerbased,real-timePCR
Downto10DNAcopies/assay.
qPCRMix,SpeciesMix,RehydrationBuffer,PCRGradeWater,InternalControl,PositiveControl
SampleRequirements
IsolatedtotalDNAfrompotentiallycontaminatedfoodserveshereasstartingmaterial,typicallyafterpre-cultivationofthesamplegrowthmedium.
PCRreactiontubes,1.5mlreactiontubes
Optional:DNAcanbeextractedfromenrichmentbrothusingtheDNAextractionkitExtractNow™FoodControl(Cat.No.609-1050)orusermethod.
qPCRcyclerwithFAM™andROX™filter
VariablemicroliterPipettes
Benchtopcentrifugefor1.5mlreactiontubes
150minutes
Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationthereagentsmustbestoredat-18°C.
No,certificationinprogress
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn