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产品资料

Minerva-biolabs/Food Control™ LFA+/11-04-12-025/

FoodControl™LFA+isaPCR-kitforfastandreliabledetectionoffoodbornepathogensviaconventionalPCRincombinationwithourreliableLateralFlowAssay(LFA)+cartrigetechnology,puttingtogethertheeasilyinterpretableLFAstripeandourPCRcontaminationavoidingLFA+cartridgesystem.

DetectingbacterialcontaminationisakeyaspectinfoodmicroBIOLOGy.Consequently,microbiologicalqualitycontrolprogramsareintensifiedthroughoutfoodchainproductioninordertominimizetheriskofintoxicatingconsumers.ReliABIlity,speedandrobustnessofatestsystemtodetectthepresenceorabsence,oreventhedegreeofcontaminationwithpathogens,becomesincreasinglyimportant.FoodControl™LFAisadiagnosticsystemforeasynon-quantitativedetectionofpresenceorabsenceoffoodbornepathogensinagriculturalorfoodindustryviaconventionalPCRandLateralFlowCartridgeLFA+technology.

TypeofAssay

FoodControl™PCR-LFA+isbasedontheregularFoodControl™PCR-LFAdesign,butprovidesadditionallyaconvenientpre-castedhybridisationcartridge.Thissystemallowsthesingle-use,cross-contaminationsafereADIngofthePCRresultforeachsampleindividually.

TheLFA+cartridgeprovidesthelateralflowstripeandtherunningbufferrequiredtocompletethehybridizationandstainingprocess.ThePCRtubeisinsertedintothecartridge.Byclosingthesystemaneedletapsthetubeandthebufferreservoir,instantlystartingtheflowandthehybridizationtransferringthePCRampliconsontothestripe.Thisonestepsystemreducespreparationandhandlingstepsaswellascontaminationviae.g.PCRcarryover.TheresultiseasilyvisIBLethroughthesightfunnelofthecartridge.

RecommendedUse/Scope

Canbeusedinresearchandindustryfordetectionofpathogeniccontaminationinfoodoragriculturalproducts.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.

ThekitisavailableforthedetectionofthefollowingmicroorganismsviaconventionalPCR:

Features

 

PackageSizes

Packagesize25reactionseach:

  • Cat.No.11-04-01-025Salmonellaenterica
  • Cat.No.11-04-02-025Yersiniaenterocolitica
  • Cat.No.11-04-03-025Shigellaspp.
  • Cat.No.11-04-04-025Campylobacterspp.
  • Cat.No.11-04-05-025Clostridiumperfringens
  • Cat.No.11-04-06-025ShigaToxin1
  • Cat.No.11-04-07-025ShigaToxin2
  • Cat.No.11-04-08-025EscherichiacoliO157
  • Cat.No.11-04-09-025EscherichiacoliO104
  • Cat.No.11-04-10-025Listeriaspp.
  • Cat.No.11-04-11-025Listeriamonocytogenes
  • Cat.No.11-04-12-025Salmonellaspp.
ResultEvaluation

LFA+cartridgeintegratedLateralFlowStripe.
Sensitivity

Downto10DNAcopies/assay.

Content

PCRMix,rehydrationbuffer,PCRgradewater,positivecontrol,speciesspecificprimer,speciesspecificprobe,LFAstripe,
LFArunningbuffer,LFA+cartridge,LFAcartridgechamber.

SampleRequirements

TheFoodControl™kitsaredesignedforthePCRbaseddetectionofbacterialDNA.IsolatedtotalDNAfrompotentiallycontaminatedfoodserveshereasstartingmaterial,typicallyafterpre-cultivationofthesamplegrowthmedium.

Timetoresult

150minutes

RequiredConsumables

0.2mlPCRreactiontubes

Optional:DNAcanbeextractedfromenrichmentbrothusingtheDNAextractionkitExtractNow™FoodControl(Cat.No.609-1050)orusermethod.

RequiredLabDevices

Racksforreactiontubes,benchtopcentrifugeforreactiontubesorPCRplates,vortex,variablemicroliterPipettes(1µl–1000µl),sterilefiltertips,anyconventionalPCRcycler.

ShelfLifeandStorage

Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationthereagentsmustbestoredat-18°C.

Compliance

No,certificationinprogress.

Micro-organismTarget
Salmonellaenterica
invasionproteinA(invA)gen
YersiniaenterocoliticaheatstableenterotoxinAgen
Shigellaspp.invasionplasmidantigen(ipaH6)gen
Campylobacterspp.acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine

O-acyltransferase(lpxA)gen

ClostridiumperfringensphospholipaseCalphatoxin(plc)gen
Shigelladysenteriae,
Escherichiacoli(EHEC)
ShigaToxin1(stx1)gen
Shigelladysenteriae,
Escherichiacoli(EHEC)
ShigaToxin2(stx2)gen
EscherichiacoliO157wBDRgen
EscherichiacoliO104wckDgen
Listeriaspp.invasionassociatedproteinp60(iap)gen
ListeriamonocytogeneslisteriolysinO(hly)gen
Salmonellaspp.spacer-regionbetween16Sand23SRNAgenes