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产品资料

Minerva-biolabs/Food Control™ LFA/11-03-12-025/

FoodControl™LFAisaPCR-kitforfastandreliabledetectionoffoodbornepathogensviaconventionalPCRincombinationwithourreliableLateralFlowAssay(LFA)evaluationtechnology.

DetectingbacterialcontaminationisakeyaspectinfoodmicroBIOLOGy.Consequently,microbiologicalqualitycontrolprogramsareintensifiedthroughoutfoodchainproductioninordertominimizetheriskofintoxicatingconsumers.ReliABIlity,speedandrobustnessofatestsystemtodetectthepresenceorabsence,oreventhedegreeofcontaminationwithpathogens,becomesincreasinglyimportant.FoodControl™LFAisadiagnosticsystemforeasynon-quantitativedetectionofpresenceorabsenceoffoodbornepathogensinagriculturalorfoodindustryviaconventionalPCRandLateralFlowAssay(LFA)evaluationtechnology.

TypeofAssay

TheassayisbasedonaonetubeamplificationwithaconventionalPCRcyclerandasubsequentprobespecifichybridizationtechnique.Theresultinginterpretationisasimplereadoutofcolouredbarsonalateralflowstripe.

RecommendedUse/Scope

Canbeusedinresearchandindustryfordetectionofpathogeniccontaminationinfoodoragriculturalproducts.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.

ThekitisavailableforthedetectionofthefollowingmicroorganismsviaconventionalPCR:

Features
PackageSizes

Packagesize25reactionseach:

  • Cat.No.11-03-01-025Salmonellaenterica 
  • Cat.No.11-03-02-025Yersiniaenterocolitica
  • Cat.No.11-03-03-025Shigellaspp.
  • Cat.No.11-03-04-025Campylobacterspp. 
  • Cat.No.11-03-05-025Clostridiumperfringens
  • Cat.No.11-03-06-025ShigaToxin1
  • Cat.No.11-03-07-025ShigaToxin2
  • Cat.No.11-03-08-025EscherichiacoliO157
  • Cat.No.11-03-09-025EscherichiacoliO104
  • Cat.No.11-03-10-025Listeriaspp.
  • Cat.No.11-03-11-025Listeriamonocytogenes
  • Cat.No.11-03-12-025Salmonellaspp.
ResultEvaluation

LateralFlowStripe.

Sensitivity

Downto10DNAcopies/assay.

Content

PCRMix,rehydrationbuffer,PCRgradewater,positivecontrol,speciesspecificprimer,speciesspecificprobe,LFAstripe,
LFArunningbuffer

SampleRequirements

TheFoodControl™kitsaredesignedforthePCRbaseddetectionofbacterialDNA.IsolatedtotalDNAfrompotentiallycontaminatedfoodserveshereasstartingmaterial,typicallyafterpre-cultivationofthesamplegrowthmedium.

Timetoresult

150minutes

RequiredConsumables

PCRreactiontubes,2.0mlreactiontubes

Optional:DNAcanbeextractedfromenrichmentbrothusingtheDNAextractionkitExtractNow™FoodControl(Cat.No.609-1050)orusermethod.

RequiredLabDevices

Racksforreactiontubes,benchtopcentrifugeforreactiontubesorPCRplates,vortex,variablemicroliterPipettes(1µl–1000µl),sterilefiltertips,anyconventionalPCRcycler.

ShelfLifeandStorage

Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationthereagentsmustbestoredat-18°C.

Compliance

No,certificationinprogress.

Micro-organismTarget
Salmonellaenterica
invasionproteinA(invA)gen
YersiniaenterocoliticaheatstableenterotoxinAgen
Shigellaspp.invasionplasmidantigen(ipaH6)gen
Campylobacterspp.acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine

O-acyltransferase(lpxA)gen

ClostridiumperfringensphospholipaseCalphatoxin(plc)gen
Shigelladysenteriae,
Escherichiacoli(EHEC)
ShigaToxin1(stx1)gen
Shigelladysenteriae,
Escherichiacoli(EHEC)
ShigaToxin2(stx2)gen
EscherichiacoliO157wBDRgen
EscherichiacoliO104wckDgen
Listeriaspp.invasionassociatedproteinp60(iap)gen
ListeriamonocytogeneslisteriolysinO(hly)gen
Salmonellaspp.spacer-regionbetween16Sand23SRNAgenes