TheAquaScreen®qPCRDetectionkitseriesisspecificallydesignedforthequantitativedetectionofcommonwaterpathogenssuchasLegionella,Pseudomonas,andE.coli.TheAquaScreen®approachincludessamplepreparationusingtheAquaScreen®FastExtractkitandvariousqPCRdetectionkits.Incontrasttotime-consumingcellculturemethods,theAquaScreen®approachneedslessthansixhoursincludingsamplepreparationandqPCRtoreliablydetectwaterpathogens.Inaddition,ourqPCRassayissuperiorintermsofsensitivityandspecificityastheassayonlydetectstherespectivespecies.TheAquaScreen®qPCRassayisinsensitivetocontaminationwithotherbacterialgenera.Also,theassay’srobustnessisunsurpassedwithlineardetectionupto3x106particlespersample.
Quantitativereal-timePCR(qPCR)
TheAquaScreen®LegionellapneumophilaqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofLegionellapneumophilainwaterssamples.TheusedprimersetisspecificforasegmentofthemipregionoftheLegionellapneumophilagenome.
ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.
Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater
PCRreactiontubes
Optional:ForquantificationwerecommendourLegionellapneumophilaPCRQuantificationStandard(Cat.No.52-0101).
qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips
Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow-18°C.
AFNORXPT90-471andISO/TS12869:2012
Quantitativereal-timePCR(qPCR)
TheAquaScreen®LegionellaspeciesqPCRDetectionKitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofLegionellainwaterssamples.Thesuppliedprimersetisspecificforabroadrangeoflegionellaspecies,butdoesnotdetectotherwaterbornebacteriaasrequiredbyISO/TS12869:2012.
ApplicableforwatertestingasdescribedinISO/TS12869:2012,inresearchandindustryforQAtestingofprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.
Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater
PCRreactiontubes
Optional:ForquantificationwerecommendourLegionellapneumophilaPCRQuantificationStandard(Cat.No.52-0101).
qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips
Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow -18°C.
AFNORXPT90-471andISO/TS12869:2012
Quantitativereal-timePCR(qPCR)
TheAquaScreen®PseudomonasaeruginosaqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofPseudomonasaeruginosainwaterssamples.
ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.
Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater
PCRreactiontubes
Optional:ForquantificationwerecommendourPseudomonasaeruginosaPCRQuantificationStandard(Cat.No.52-0071).
qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips
Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow -18°C.
Quantitativereal-timePCR(qPCR)
TheAquaScreen®EscherichiacoliqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofEscherichiacoliinwaterssamples.
ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.
Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater
PCRreactiontubes
Optional:ForquantificationwerecommendourE.coliPCRQuantificationStandard(Cat.No.52-0083).
qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips
Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow -18°C.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn