FLIPR膜电位检测试剂盒 Molecular Devices

TheFLIPR®MembranePotentialAssayKitisahomogeneousno-washassaywithmixandreadsimplicity,rapidreadtimes,andgreatertemperaturestABIlityforextendedhighthroughputionchannelscreeningapplications.Twodifferentno-washformulationsareavailableforeachkit,andusesaproprietaryindicatordyecombinedwithdifferentquencherstomaximizecellline/channel/compoundapplicabilitywhileeliminatingcausesofvariabilityinthedata.Theproprietarydyeshave10timesfasterresponsetimesandgreatertemperaturestabilitythantrADItionaldyessuchasDiBAC,whichenablesbatchprocessingofscreeningplatesorextendedautomatedruns.Inaddition,unlikeDiBACwhichcanonlyshowunidirectionalchangestomembranepotential,theFLIPRMembranePotentialAssayKitcandetecttheopeningandclosingoftheionchannel.Proprietaryquenchtechnologyreducesbackgroundfluorescenceforawidersignalwindowandprovidesthebestsignal-to-noiseratio.TheFLIPRMembranePotentialAssayKitdelivershigherthroughputthantraditionalautomatedFLIPRionchannelprotocolswhileprovidingdatathatshowsgoodcorrelationwithmanualpatchclampassays.

 

FLIPRMembranePotentialAssaykitsdetectionchannelmodulationbyincreasingordecreasingthefluorescentsignalascellularmembranepotentialchanges.Thefluorescentsignalincreasesinintensityduringmembranedepolarizationasdyefollowsthepositivelychargedionsinsidethecell.Duringmembranehyperpolarization,fluorescentsignaldecreasesinintensityasdyefollowsthepositivelychargedionsoutofthecell.FLIPRMembranePotentialAssayKitsareuniquelysuitedforusewiththesimultaneouspipetandreadcapabilityoftheFLIPR^TETRA®systemtocapturethefastkineticsassociatedwiththisassay.

 

Becauseionchannelactivityissensitivetointerference,andchemicalinterferencewithaparticularionchannelishighlyunpredictable,theFLIPR^®MembranePotentialAssayKithastwoformulations,blueandred. BothformulationscombinetheadvantagesofMolecularDevicesproprietarymembranepotentialindicatordyewithourpatentedquenchtechnology. Werecommendthatbothredandblueversionsbeevaluatedforeachindividualionchanneltargettodeterminewhichformulationwillprovideoptimalperformance.

 

FLIPRAssayKitsfromMolecularDevicesemployaquenchingdyetoreducebackgroundfluorescenceandimprovethesignal-to-noiseratio.Thepatentedquenchtechnology(U.S.patentnumber6,420,183,EPOpatentnumber0906572)isofferedtodrugdiscoveryandlifescienceresearchersexclusivelybyMolecularDevices,throughthepurchaseofFLIPRAssayKits.