产品名称：CHOLERATOXINBSUBUNIT, 霍乱毒素B亚单位
化学名：Choleragenoid;CTB；CTxB
CASNo：131096-89-4
分子式：
分子量：
纯度：≥95%(SDS-PAGE)
溶解度：H2O:soluble1mg/mL(stableindefinitelyat2-8°C),Reconstitutewithwatertoafinalconcentrationof
1mgcholeratoxinBsubunitperml.Swirlbottlesgentlyduringreconstitution.Avoidvigorouspipetting
ofsolutionsthatmayleadtofoaming.Solutionscanbefilteredthrougha0.2mmfilter.
保存：2-8C,Theproduct,assupplied,isstable2yearswhenstoredproperly.Solutionsarestableforatleast3
monthswhenstoredat2–8C.DONOTFREEZE.
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货号：abs80001
规格：大包装询价
货号产品名称规格
abs80001 CHOLERATOXINBSUBUNIT 1mg
库存：现货
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生物活性：
CholeratoxinbelongstotheAB5–subunitfamilyoftoxins.Thenativehexamericproteinhasamolecularmassof
~85kDaandcontainstwosubunits.ItconsistsofasingleAsubunit(~27.2kDa),respons
IBLeforthe
ADP-ribosylationactivity,andfiveBsubunits(~11.6kDaeach),whicharearrangedasapentamericringwithan
apparent5-foldsymmetryandareassociatedwiththecellsurfacereceptorbindingandsubsequent
internalization(transmembranetransport)oftheenzymaticcomponent.
CholeratoxinanditspurifiedAsubunitarefrequentlyusedforthestudyofsignaltransductionmechanisms.In
addition,choleratoxinactsasanadjuvantthroughthestimulationofBlymphocytes.ThecholeratoxinBsubunit
aloneisusedfortracktracinginneurologicalresearch,takingadvantageofGM1gangliosidebindingand
retrogradetransport.Tissueculturecellstreatedwithcholeratoxinarenotkilledandtissuesofanimalsdonot
becomenecrotic.
TheBsubunitisnon-toxictocellsandpossessesnointrinsicadenylatecyclaseactivity.ThecholeratoxinB
subunit(CTB)attachestocellsbybindingtogangliosideGM1.8CTBhasbeenshowntobeagoodlabelfor
microglialcells(duetotheenrichmentofgangliosideGM1ontheircellsurface),butnotforoligodendrocytesor
astrocytes.TheBsubunithasbeenreportedtobeanexcellenttracerforthestudyofaxonaltransportusing
immunohistochemicalmethods.Recentlyithasbeenwidelyusedasa
Markerofmembranelipidrafts,whichare
membranemicrodomainsenrichedwithcholestrolandsphingolipids.Theselipidraftshaveanimportantrolein
cellsignalingandproteintrafficking.
