- J. F. Hainfeld, et al., Microsc. Microanal. 8 (Suppl. 2) (2002) 916 CD.
- R. Tubbs, J. Pettay, et al. J. Mol. Histol. 35 (2004) 589.
- R. Tubbs, J. Pettay, J., et al. Appl. Immunohistochem. Mol. Morphol. 13 (2005) 371.
- A. Cali & P. M. Takvorian, Southeast Asian Trop. Med. Public Health, 35 (Suppl. 1) (2004) 58.
- A. Cali, L. M. Weiss, and P. M. Takvorian, J. Euk. Microbiol., 49 (2003) 164.
- E.M. Keohane, G.A. Orr, et al., Mol. Biochem. Parasitol., 94 (1998) 227. This work was supported by NIH SBIR grant 2R44 GM064257-02A1 and NIH grant 2R01 AI031788. The authors thank Ventana Medical Systems, Incorporated for the I-View reagent.
Ni-NTA纳米金 – 特异性结合his标签蛋白，用于标记his标签蛋白：
应用：
透射电镜/扫描透射电镜、光学显微镜、蛋白杂交、纯化his标签蛋白时，识别含目的蛋白的部分等实验。
优点：
灵敏度高
探针小，分辨率高，1.8 nm Ni-NTA-纳米金比抗体探针小
高溶解度，稳定
无背景
永久染色
5 nm Ni-NTA-纳米金在电镜下可见，不需金增强/银增强
使用简单
左：1.8nm Ni-NTA-纳米金与his标签蛋白相互作用右：用Ni-NTA-纳米金标记6xhis腺病毒Knob蛋白，未染色的扫描电镜照片
left: Structure of Ni-NTA-Nanogold® showing interaction with Interaction with a His-tagged protein; right: Knob protein from adenovirus cloned with 6x-His tag, labeled with Ni-NTA-Nanogold, column purified from excess gold, and viewed in the scanning transmission electron microscope (STEM) unstained (Full width approximately 245 nm).
![[Ni-NTA-5 nm Gold structure, and TEM View (141k)]](http://img.ebiomall.cn/images/201459153347173.jpg)
上：5nm Ni-NTA-纳米金的结构，显示金属螯合物与his标签蛋白结合
下：5nm Ni-NTA-纳米金的扫描电镜图，平均直径5.11±0.84nm。
Top: Structure of NTA-Ni(II)-5 nm Nanogold®, showing the binding of the incorporated metal chelate to a His-tagged protein; distance from the gold particle surface to the His tag is estimated to be 1.5 nm. Above: Transmission electron micrograph of 5 nm NTA Nanogold: average diameter 5.11±0.84nm.
品名 | 产品描述 | 货号 | 规格 |
1.8 nm Ni-NTA-Nanogold® | 比抗体小，更易渗透入组织，定位和检测细胞、组织或蛋白复合物中的his标签蛋白。 | 2080 | 10uM 30ml |
5 nm Ni-NTA-Nanogold® | 目标量达一定程度时，无需银增强/金增强可直接用电镜观察 | 2082 | 0.5uM 3ml |
相关产品：
GoldEnhance LM/Blot (GELM) | 金增强试剂，用于光镜样品 | 2112 | Initiator/Moderator/Activator/Buffer
各15 ml（共60 ml，足够做600张载玻片） |
GoldEnhance EM(GEEM) | 金增强试剂，用于电镜样品 | 2113 | Initiator/Moderator/Activator/Buffer
各2 ml（共8 ml，足够做200个网格） |
HQ Silver | 用于纳米金的质量**的增强试剂，均匀显影，极好的保持结构，特别适合电镜，光敏感 | 2012 | Initiator/Moderator/Activator
各15 ml（共45 ml） |
Li Silver | 纳米金的银增强，用于电镜、光镜、凝胶、杂交，光不敏感 | 2013 | Initiator/Enhancer
125 ml （共250 ml） |
References：
1. Kollman, J. M.; Zelter, A.; Muller, E. G.; Fox, B.; Rice, L. M.; Davis, T. N., and Agard, D. A.: The Structure of the gamma-Tubulin Small Complex: Implications of Its Architecture and Flexibility for Microtubule Nucleation. Mol. Biol. Cell, 19, 207-215 (2008).
2. Adami, A.; Garcia-Alvarez, B.; Arias-Palomo, E.; Barford, D., and Llorca, O.: Structure of TOR and its complex with KOG1. Mol. Cell., 27509-516 (2007).
3. Balasingham, S. V.; Collins, R. F.; Assalkhou, R.; Homberset, H.; Frye, S. A.; Derrick, J. P., and Tonjum, T.: Interactions between the Lipoprotein PilP and the Secretin PilQ in Neisseria meningitidis. J. Bacteriol., 189, 5716-5727 (2007).
4. Jiang, Z. G.; Simon, M. N.; Wall, J. S., and McKnight, C. J.: Structural analysis of reconstituted lipoproteins containing the N-terminal domain of apolipoprotein B. Biophys. J., 92, 4097-4108 (2007).
5. Pye, V. E, Beuron, F, Keetch, C. A, McKeown, C, Robinson, C. V, Meyer, H. H, Zhang, X, and Freemont, P. S.: Structural insights into the p97-Ufd1-Npl4 complex. Proc. Natl. Acad. Sci. USA, 104, 467-472 (2007).
6. Promnares, K.; Komenda, J.; Bumba, L.; Nebesarova, J.; Vacha, F., and Tichy, M.: Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits. J. Biol. Chem., 281, 32705-32713 (2006).
7. Collins, R. F.; Beis, K.; Clarke, B. R.; Ford, R. C.; Hulley, M.; Naismith, J. H.; and Whitfield, C.: Periplasmic protein-protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules. J. Biol. Chem.,281, 2144-2150 (2006).
8. Wolfe, C. L.; Warrington, J. A.; Treadwell, L., and Norcum, M. T.: A three-dimensional working model of the multienzyme complex of aminoacyl-tRNA synthetases based on electron microscopic placements of tRNA and proteins. J. Biol. Chem., 280, 38870-38878 (2005).
9. Bumba, L.; Tichy, M.; Dobakova, M.; Komenda, J., and Vacha, F.: Localization of the PsbH subunit in photosystem II from the Synechocystis 6803 using the His-tagged NiNTA Nanogold labeling. J. Struct. Biol., 152, 28-35 (2005)
10. Collins, R. F.; Frye, S. A.; Balasingham, S.; Ford, R. C.; Tonjum, T., and Derrick, J. P.: Interaction with type IV pili induces structural changes in the bacterial outer membrane secretin PilQ. J. Biol. Chem., 280, 18923-18930 (2005).
11. Chatterji, A.; Ochoa, W. F.; Ueno, T.; Lin T., and Johnson, J. E.: A virus-based nanoblock with tunable electrostatic properties. Nano Lett.,5, 597-602 (2005).
12. Buchel, C.; Morris, E.; Orlova, E., and Barber, J.: Localisation of the PsbH subunit in photosystem II: a new approach using labelling of His-tags with a Ni(2+)-NTA gold cluster and single particle analysis. J. Mol. Biol., 312, 371-379 (2001).
13. Hainfeld, J. F.; Liu, W.; Halsey, C. M. R.; Freimuth, P., and Powell, R. D.: Ni-NTA-Gold Clusters Target His-Tagged Proteins. J. Struct. Biol., 127, 185-198 (1999).
14. Hainfeld, J. F.; Liu, W.; Joshi, V., and Powell R. D.: Nickel-NTA-Nanogold Binds his-Tagged Proteins. Microsc. Microanal., 8, (Suppl. 2: Proceedings) (Proceedings of Microscopy and Microanalysis 2002); Voekl, E.; Piston, D.; Gauvin, R.; Lockley, A. J.; Bailey, G. W., and McKernan, S., Eds.; Cambridge University Press, New York, NY, 2002, p. 832CD.
GoldiBlot™ HIS Western Blot Kit：Ni-NTA-纳米金颗粒
应用：
1.蛋白杂交检测带his标签的重组蛋白（染色时间1小时）
2.识别细胞裂解液或提取物中的his标签蛋白
3.证实转染细胞中his标签蛋白的表达
优点：
更快更灵敏
低背景
永久信号
![[GoldiBlot Western Blot (43k)]](http://img.ebiomall.cn/images/201459153429915.jpg)
品名 | 产品描述 | 货号 | 规格 |
GoldiBlot™ HIS Western Blot Kit | 用于在蛋白杂交中检测his重组蛋白的 | 2090 | 15个杂交 |
References：
- Dubendorff, J.; Cruz, M.; Gonzalez, C.; Hainfeld, J.; Liu, W.: Rapid Detection of His-tagged Proteins on Western Blots Proc. 47th Ann. Mtg., Amer. Soc. Cell Biol., 47; Pres. # 1918., poster # B265 (2007).
![[EnzMet vs. DAB: ISH of HER2 gene in amplified tissue (36k)]](http://img.ebiomall.cn/images/20145915311385.jpg)
![[EnzMet - ISH mechanism (31k)]](http://img.ebiomall.cn/images/201459153130909.jpg)
![[ (93k)]](http://img.ebiomall.cn/images/201459153522181.jpg)