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Rabbitpolyclonalantibody
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofratGAT-1.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviachromatographywithacolumnmadewiththepeptideusedasantigen.
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAAreceptor(GABAA-R)subtype.GABAplasmamembranetransporters(GATs)influencesynapticneurotransmissionbyhighaffinityuptakeandreleaseofGABA.Todate,fourdistinctGABAtransportershavebeenidentified:GAT-1,GAT-2,GAT-3,andBGT-1.GAT-1,themostabundantofthetransporters,isfoundpredominantlyinneurons,butalsoinsomespecializedglia(Minellietal.,1995).GAT-1isthoughttoplayakeyroleinepileptogenesis(Zhaoetal.2003).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Johnson,J.,Chen,T.K.,Rickman,D.W.,Evans,C.,&Brecha,N.C.(1996).Multipleγ-aminobutyricacidplasmamembranetransporters(GAT-1,GAT-2,GAT-3)intheratretina.TheJournalofcomparativeneurology,375(2),212.PMID:8915826