Macrophage depletion improves survival of porcine neonatal pancreatic cell clusters contained in alginate macrocapsules transplanted into rats. PubM

Xenotransplantation2003May;10(3):240-51.全文索取Macrophagedepletionimprovessurvivalofporcineneonatalpancreaticcellclusterscontainedinalginatemacrocapsulestransplantedintorats.
OmerA,KeeganM,CzismADIaE,DeVosP,VanRooijenN,Bonner-WeirS,WeirGC.

Abstract
Macrophagescanaccumulateonthesurfaceofemptyandislet-containingalginatecapsules,leadingtolossoffunctionaltissue.Inthisstudy,theeffectofperitonealmacrophagedepletiononthebiocompatibilityofalginatemacrocapsulesandfunctionofmacroencapsulatedporcineneonatalpancreaticcellclusters(NPCCs)wasinvestigated.ClodronateliposomeswereinjectedintotheperitonealcavitiesofnormoglycemicLewisrats5and2daysbeforethetransplantation.EmptyorNPCC-containingCa-alginatepolyL-lysine(PLL)-coatedmacrocapsulesweretransplantedintotheperitonealcavitiesofratsinjectedwitheitherclodronateliposomesorsaline.Ondays7,14and21,sampleswereevaluatedbyimmunohistochemistryforcellularimmuneresponsesonthesurfaceofthemacrocapsulesandformacrophagepopulationsinomentaltissue.ToassessthefunctionofmacroencapsulatedNPCCs,insulinsecretoryresponsestoglucoseandtheophyllineweremeasuredaftercapsuleretrieval.Insaline-injectedcontrolgroups,alloftheemptyandNPCC-containingmacrocapsuleswereovergrownwithmacrophages,thisbeingespeciallysevereonNPCC-containingmacrocapsules.Intheclodronateliposomes-injectedgroup,themajorityoftheemptymacrocapsuleswerefreeofmacrophageaccumulationandtheNPCC-containingmacrocapsuleswerelessovergrownthanincontrolanimals.HigherinsulinresponsestoglucoseandtheophyllinewereobservedinNPCCsretrievedfromratsinjectedwithclodronateliposomes.WeconcludethatdepletionofperitonealmacrophageswithclodronateliposomesimprovethesurvivalofmacroencapsulatedNPCCs.

PMID:12694544[Pubmed-MEDLINE]