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| 货号： | DL-SERT-Mu |
| 样本： | 血清，血浆，尿液细胞裂解液，组织匀浆，细胞培养上清液 |
| 标记物： | 详见说明书 |
| 应用： | ELISA定量检测 |
| 检测方法： | 夹心法 |
| 检测限： | 详见说明书 |
| 供应商： | 康朗生物 |
| 数量： | 大量供应 |
| 规格： | 96T/盒 |
| Productname: | MouseSerotoninTransporter(SERT)ELISAKit |
| Method: | sandwich |
| Synonyms: | SLC6A4;SLC6-A4;HTT;5-HTT;5HTT;OCD1;hSERT;SoluteCarrierFamily6,Member4;NeurotransmitterTransporter,Serotonin;Sodium-dependentserotonintransporter |
| Catalognumber: | DL-SERT-Mu |
| Detectionrange: | 0.156-10ng/mL |
| Size: | 96T |
| Assaylength | 1-4.5Hours |
| Price: | inquiry |
| Qualityguaranteeperiod: | for12months |
| Item | Standard | TestResult | |
| Description | Thisimmunoassaykitallowsforthespecificmeasurementofthisindex inserum,Plasma,Urine,tissuehomogenatesandCellculturesupernatesandOtherBIOLOGicalfluids.. | Conform | |
| Identification | Colorimetric | Positive | |
| Composition | Pre-coated,readytouse96-wellstripplate Standard(freezedried) StandardDiluent DetectionReagentA DetectionReagentB AssayDiluentA AssayDiluentB TMBSubsthumane StopSolution WashBuffer(30xconcenthumane) Platesealerfor96wells Instructionmanual | 1 2 1×20ml 1×120μl 1×120μl 1×12ml 1×12ml 1×9ml 1×6ml 1×20ml 2 1 | Conform |
Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictothisindex.Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedpolyclonalantibodypreparationspecificforthisindexandAvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.ThenaTMBsubstratesolutionisaddedtoeachwell.Onlythosewellsthatcontaintheindex,biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±2nm.TheconcentrationoftheindexinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve
Matriceslistedbelowwerespikedwithcertainlevelofrecombinanttheindexandtherecoveryhumaneswerecalculatedbycomparingthemeasuredvaluetotheexpectedamountoftheindexinsamples.
| Matrix | Recoveryrange(%) | Average(%) |
| serum(n=5) | 81-93 | 86 |
| EDTAplasma(n=5) | 80-97 | 88 |
| heparinplasma(n=5) | 90-101 | 95 |
Thelinearityofthekitwasassayedbytestingsamplesspikedwithappropriateconcenthumanionoftheindexandtheirserialdilutions.Theresultsweredemonsthumanedbythepercentageofcalculatedconcenthumaniontotheexpected.
| Sample | 1：2 | 1：4 | 1：8 | 1：16 |
| serum(n=5) | 82-96% | 83-98% | 81-99% | 93-101% |
| EDTAplasma(n=5) | 88-101% | 86-95% | 90-102% | 80-93% |
| heparinplasma(n=5) | 80-91% | 82-90% | 95-104% | 79-95% |
Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighleveltheindexweretested20timesononeplate,respectively.
Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighleveltheindexweretestedon3differentplates,8replicatesineachplate.
CV(%)=SD/meanX100
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.
Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend
1.Prepareallreagents,samplesandstandards;
2.Add50µLstandardorsampletoeachwell.
Andthenadd50µLpreparedDetectionReagentAimmediately.
Shakeandmix.Incubate1hourat37℃;
3.Aspirateandwash3times;
4.Add100µLpreparedDetectionReagentB.Incubate1hourat37℃;
5.Aspirateandwash5times;
6.Add90µLSubstrateSolution.Incubate15-25minutesat37℃;
7.Add50µLStopSolution.Readat450nmimmediately.
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