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| Description |  Glucosetransporter1(GLUT1)isamulti-passmembraneprotein.Itisamemberofthefamilyofglucosetransportersthatfacilitateglucoseuptakebycells.GLUT1isamajortransporteratthemammalianblood-brainbarrier. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(FYNQTWVHRYGESI)correspondingtoaregion(43-56)fromtheN-terminusofhumanGlucosetransporter1(GLUT1). |
| OtherNames | SLC2A1;GLUT-1; |
| Accession | P11166GTR1_HUMAN |
| Producedin | Rabbit |
| Purity | Affinitypurifiedonantigencolumn |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.HumanGLUT1hasapredictedlengthof492residuesandMWof55kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectGLUT1informalinfixedandparaffinembeddedtissues.Antigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;mouse;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1µg/µl.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |