Agrisera/NRT1.1 | Nitrate transporter 1.1/AS12 2611/


productinformation
Background		NRT1.1(nitratetransporter1.1) isadual-affinitynitratetransporterwhichactsalsoasanitratesensor.High-affinitynitratetransporterwhenphosphorylatedandlow-affinitytransportedwhendephosphorylated.Lownitrogenconcentrationstimulatesphosphorylation.Expressedinlateralroots. Alternativenames:AtNRT1,Nitrate/chloratetransporter,proteinCHLORINA1,AtNPF6.3.
Immunogen		KLH-conjugatedsyntheticpeptidederivedfromArabidopsisthaliana NRT1.1,UniProt:Q05085,TAIR:AT1G12110
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinitypurifiedserum
Format		LyophilizedinPBSpH7.4
Quantity		50µg
Reconstitution		Forreconstitutionadd50µlofsterilewater.
Storage		storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.
Testedapplications		westernblot(WB),immunolocalization(IL)
Relatedproducts		AS122612\|NRT2.1\|nitratetransporter2.1,rabbitantibody Plantproteinextractionbuffer Secondaryantibodies
Additionalinformation

applicationinformation
Recommendeddilution		1:5000withstandardECL(WB)
Expected\|apparentMW		64kDa
Confirmedreactivity		Arabidopsisthaliana
Predictedreactivity		Brassicanapus
Notreactivein		noconfirmedexceptionsfrompredictedreactivityarecurrentlyknown
Additionalinformation		tobeaddedwhenavailable
Selectedreferences		tobeaddedwhenavailable,antibodyreleasedinMay2014.

applicationexample

50µgofmicrosomalproteinfromwildtypeArabidopsisthaliana(Col0)rootsatvariousstagesofdevelopmentorfromNRT1.1deletionmutant(CHL1.5)weresolubilizedwithLaemmlix2samplebufferandwereseparatedon10%SDS-PAGEgel.Theproteinsweretransfered(tanktransfer,100V,1h)toaPVDFmembrane(Immobilon-P,0.45um,Millipore).Blotwasblockedfor2hwithagitationatroomtemperatureinblockingbuffer(SuperBlock,Thermo)containing0.05%Tween20(Thermo).Blotwasthenincubatedfor2hatRTandwithagitationinthefreshblockingbuffercontainingtheprimaryantibodyatadilutionof1:5000.Theantibodysolutionwasdecantedandtheblotwaswashed3timesfor10mininPBS-T(0.05%Tween20,Thermo)atRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGhorseradishperoxidaseconjugated,fromAgrisera,AS09602)dilutedto1:10000inblockingbuffer,for2hatRTwithagitation.Theblotwaswashed1timefor10mininPBS-Tand2timesfor10mininPBS.TheblotwasthenincubatedwithECLreagent(SuperSignalWestPico,Thermo)for5minandthechemioluminescencewasrecordedonLAS3000,Fuji.Exposuretimeswerebetween1-10minusingstandard(lowest)sensitivity.

CourtesyofDr.WojciechSzponarski,FrenchNationalInstituteofAgriculturalResearch,France

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Agrisera/NRT1.1 | Nitrate transporter 1.1/AS12 2611/