QED Bioscience/VGlut2 Transporter/56515/1 Ea

CatalogNo.:56515(cloneS29-29)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.

PurifiedbyProteinGaffinitychromatography.

TheATP-dependent,chloride-sensitivevesicularglutamatetransportersincludeVGlut1,VGlut2,andVGlut3.VGlut1andVGlut2areexpressedinbraininacomplementaryfashion.VGlut1isexpressedinthetelencephalicregionswhereasVGlut2isexpressedinthelowerbrainstemanddiencephalicregions.

VGlut3isexpressedinasmallnumberofneuronsinthestriatum,hippocampus,cerebralcortex,andraphenuclei.

PancreaticαandβcellsexpressVGlut1andVGlut2inresponsetoglucoseconcentrations.

Antigen:Fusionproteincorrespondingtoaa501-582(cytoplasmicC-terminus)ofratVGlut2(accessionno.NP_445879).

HostSpecies:Mouse

AntibodyClass:IgG1

Thisantibodyrecognizeshuman,mouse,andratVGlut2.

Immunoblotting:useat1-2ug/ml.Abandof~60kDaisdetected.

Adultratbrainimmunoblot

Immunohistochemistry:useat1-5ug/ml.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:ratbrainlysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisproductisstableforatleast1yearat

-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.