企业QQ
官方微信
Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr1007ofmouseKCC2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-andnon-phosphopeptideaffinitycolumns.
KCC2iswidelythoughttobeexpressedexclusivelyinneuronswhereitisresponsibleformaintaininglowintracellularchlorideconcentrationtodrivehyperpolarizingpost-synapticresponsestotheinhibitoryneurotransmittersGABAandglycine(Leeetal.,2007).KCC2isexpressedinmostadultneurons,andexpressionlevelscorrelatewellwiththematurationstateofneurons.N-ethylmaleimide(NEM)hasbeenshowtoincreasephosphorylationoftheSer940residue,whiledecreasingphosphorylationofT1007residue.DephosphorylationofresiduesT906andT1007correlateswithincreasedKCC2activity(Mossetal.,2017).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Conway,L.C.,Cardarelli,R.A.,Moore,Y.E.,Jones,K.,McWilliams,L.J.,Baker,D.J.,Burnham,M.P.,Bürli,R.W.,Wang,Q.,Brandon,N.J.andMoss,S.J.,2017.N-ethylmaleimideincreasesKCC2activitybymodulatingtransporterphosphorylation.JournalofBiologicalChemistry,pp.jbc-M117.