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Neuromics/5HT (Serotonin) Transporter/RA24330/100 ul

Type:RabbitIgG

 

Applications:IHC;WB

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

SpeciesReactivity:R

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

Format:Wholeserum-lyophilized

 

Immunogen:syntheticpeptidesequencecorrespondingtoaminoacids579-599ofrat5HTtransportercoupledtoKLH.

 

Picture

Theserotonintransporter(SERT)isamonoaminetransporter,integralmembraneproteinthattransportstheneurotransmitterserotoninfromsynapticspacesintopresynapticneurons.ThistransportofserotoninbytheSERTproteinterminatestheactionofserotoninandrecyclesitinasodium-dependentmanner.

Thehistochemicalantibodyforserotonin(5HT)transporterisgeneratedinarabbitagainstasyntheticpeptidesequencecorrespondingtoaminoacids579-599ofrat5HTtransportercoupledtoKLH.

Theantibodyproduces4+(maximum)labelingof5HTtransporteratdilutionsof1/200-1/400usingindirectimmunofluorescenceandatdilutionsof1/10,000-1/15,000usingbiotin/streptavidinHRPtechniqueinratraphenuclei,hypothalamus,cortex,andspinalcord.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.ByWesternblotanalysisusingratbrainextractsofcortex,hypothalamus,midbrain,andhindbrain,theantibodyspecificallylabelsasingleband.Immunolabelingiscompletelyabolishedbypre-adsorptionwithsyntheticrat5HTtransporter.


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