eBioscience Super Bright 600 染料档案

SuperBright600是串联染料，由SuperBright436和受体染料串联而成。

► 激发光：405nm

► 发射光：600nm

► 可替代染料：eVolve605，Violet605(BV605

► 检测滤片：610/20bandpass

档案详细资料：
1.SuperBright436vsBrilliantViolet605：亮度与BV421相当，尤其适合低丰度表达的抗原。
 

SuperBright600antibodyconjugatesoffersimilarbrightnesstoBrilliantViolet605-conjugatedantibodies.(A)HumanperipheralbloodcellswerestainedwithAnti-CD3(cloneOKT3)conjugatedtoSuperBright600(redhistogram)orBrilliantViolet605(greyhistogram),usingthesameconcentrationofantibody.(B)MousesplenocyteswerestainedwithAnti-CD8a(clone53-6.7)conjugatedtoSuperBright600(redhistogram)orBrilliantViolet™605(greyhistogram),usingthesameconcentrationofantibody.

2.SuperBright436vseVolve605：亮度比eVolve605要高。

Humanperipheralbloodlymphocyteswerestainedwith Anti-CD3(cloneOKT3)conjugatedtoSuperBright600(redhistogram)oreVolve605(bluehistogram),usingthesameconcentrationofantibody.

3.SuperBright600光稳定性

(A)MousesplenocyteswerestainedwithAnti-CD4(cloneRM4-5)SuperBright600andwereeitheranalyzedimmediately(redhistogram),leftinthedarkovernight(bluehistogram),orleftexposedtolightovernight(greenhistogram). (B)HumanperipheralbloodcellswerestainedwithAnti-CD8a(cloneRPA-T8)SuperBright600andwereeitheranalyzedimmediately(redhistogram),leftinthedarkovernight(bluehistogram),orleftexposedtolightovernight(greenhistogram).

4.SuperBright436固定后的稳定性

StABIlitystudiesindicatethatSuperBright600exhibitsaminimallossoffluorescencewhencellsareexposedtovariousfixativesMouselymphocyteswerestainedwithAnti-CD45R/B220(cloneRA3-6B2)SuperBright600and:

(A)wereleftunfixed(redhistogram),orfixedinICFixationbufferfor30minutes(bluehistogram),24hours(orangehistogram),orthreedays(greenhistogram),followedbyawashinPermeabilizationbuffer.

(B)wereleftunfixed(redhistogram),orfixedinFoxp3/TranscriptionFactor/Permeabilizationbufferfor30minutes(bluehistogram),24hours(orangehistogram),orthreedays(greenhistogram),followedbyawashinPermeabilizationbuffer.

(C):wereleftunfixed(redhistogram),orfixedinICFixationbufferfollowedby90%methanolfor30minutes(bluehistogram),24hours(orangehistogram),orthreedays(greenhistogram),followedbyaFlowCytometryStainingBufferwash.

5.多色分析示例-SuperBright600.

NormalhumanperipheralbloodcellswerealiquotedinthepresenceofSuperBrightStainingBuffer(Cat.No.SB-4400),thensurfacestainedwiththeindicatedreagents.Viablecells,asdeterminedbystainingwithFixableViabilityDyeeFluor®780,wereusedforanalysistodiscriminatevariousTcellsubsets.