FAQs: Beta Amyloid Peptides, Tau Proteins, Synucleins | rPeptide

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FAQsAllFAQsGeneralCanwereceivediscountsonrPeptideproducts?Youmaybeeligibleforadiscountwhenplacingabulkorder.Pleasecontactustorequestaquotationbeforeplacingyourorder.CanyoulabelapurifiedpeptidethatIprovide?Unfortunately,itwouldnotbeanoptionforustolabelpurifiedproteinsthatyouprovide.Thelabelingoftheproteinhastooccurastheproteinisbeingproduced;wegiveit15Nnitrogensource.Ifyouwouldbeinterestedinhavingusproducealabeledpeptideorifyouhaveanyfurtherquestionspleaseletusknow.HowcanIachievebatchtobatchconsistencyofPhysicalandChemicalProperties?Duetothefactthatourbeta-amyloidsarerecombinantlyproduced,thereisabatchtobatchconsistencyinthephysicalandchemicalpropertiesofallourrecombinantbeta-amyloidpeptides.HowcanIfindrPeptide"scitations?YoumayfindrPeptide"scitationsunderthePublicationstab.CitationsarelistedbyProduct,thenbyyear.Howcanyourproductsbeused?Ourproductsarenotforhumanuse,theyareforresearchpurposesonly.HowmayIrequestapricequotationforacustomproduct?Youmayemailthequantity,purityandsequenceyouwouldliketoinfo@rpeptide.com.WhatifIcannotfindtheanswertomyquestiononline?Pleasefeelfreetoemailusattechsupport@rpeptide.comforotherinquires.Whydoessolubilityvaryfromproducttoproduct?Thekeyfactorthatdeterminesthesolubilityofourproductsistheaminoacidsequenceofeachone.Inthepreparationofourproducts,weanalyzetheaminoacidsequencesandprepareourproductsinbuffersthattheysolubilizeinreadily.Whymustsomeproductsshipondryice?Someproductsmustbestoredat-70°Candthereforecannotshipatroomtemperature.Tomaintaintheintegrityoftheproduct,itwillshipondryice.Additionalchargeswillapplyfordryiceshipments.OrderingHowcanIplaceanorder?YoucansendyourorderbyFax(678-753-0746)orcallandplacetheorderbyphone(678-753-0747)ordoitonlinehttp://www.rpeptide.com/ordering.php.Whenplacinganorderpleasegiveusthefollowinginformation.Company’s,University’sorInstitute’sNameContactPerson’sName(Attn:to)PhoneNo.FaxNo.e-mailAddressPurchaseOrderNo.CreditCardNo.ShipToAddressBillToAddressVATNo.ifneededQuotationNo.ifneededHowdoyoushipproducts?DomesticordersareshippedFedExStandardOvernight.InternationalordersshipusingFedExInternationalPriority.Howlongdoesittaketoreceivetheproducts?DomesticordersareshippedFedExStandardOvernight.InternationalordersshipusingFedExInternationalPriority.Atrackingnumberwillbeprovidedtoyousothatyoumaymonitoryourpackage.Whatisthecostofshippingondryice?Inadditiontoregularshippingcharges,dryicechargesare$25forDomesticorders,and$45forInternational.Whatisthecostofshipping?DomesticordersareshippedFedExStandardOvernightfor$40.Internationalorders(includingCanada)shipusingFedExInternationalPriorityfor$85.Additionalchargesmayapplyifdryiceisrequired.BetaAmyloidsHowcanyourBetaAmyloidsbeused?Ourproductsarenotforhumanuse,theyareforresearchpurposesonly.Howpureareyourrecombinantbeta-amyloidpeptides?Thepurityofourrecombinantbeta-amyloidpeptidesisconsistently>97%.Thesaltcontentisca.10%ofthetotalweight.Thepeptideamountspecifiedisthenetpeptidecontent.ThepeptidequantitiesareestimatedbyHPLCagainstacalibrationcurvederivedfromquantitiesestimatedbyaminoacidanalyses.HowshouldyourBetaAmyloidbestoredafterreconstitutioninammoniumhydroxide?Afterreconstitutioninammoniumhydroxide,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.orlongerterm,itsbesttolyophilizeit.HowshouldyourlyophilizedBetaAmyloidbestored?ThelyophilizedBetaAmyloidcanbestoredforatleast2yearsat-80°C.IseeyouuseHPLCtocheckpurity.Whenyousayitis>97%,isitstrictlybasedonthepeakareaofthetargetpeak?PurityisdeterminedbytheratiooftheBetaAmyloidpeakareaagainstthetotalpeakareaofallthepeakareascombinedWhatdoesHFIPstandfor?HFIPisHexafluoroisopropanolWhatisthesolubilityofyourrecombinantBetaAmyloids?Beta-amyloidpeptides:ResUSPendin1%NH4OH,ataconcentrationof1mg/ml.Sonicatefor30secondsafterithasgoneintosolution.TheBeta-Amyloid,NaOHsalts(A-1155andA-1165,http://www.rpeptide.com/products/products_7.html)canberesuspendedinwater.WhatisthestABIlityofyourlyophilizedBetaAmyloid?ThelyophilizedBetaAmyloidisstableforatleast2yearsat-80°C.Afterreconstitutioninammoniumhydroxide,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.orlongerterm,itsbesttolyophilizeit.Whenyoustatethatthisvialcontains0.5mgofpeptide,isthatpreciselyweightedorisitpossIBLethatthereisanexcesspeptide?Whenwereconstitutethepeptideanddoseitbyitsabsorbanceat280nm,wegetmuchmorepeptidethanexpected(morethan200%).Isthisnormal?Thepeptideamountiscalibratedchromatographicallyagainstknownamountsoftheproductasdeterminedbyaminoacidanalysis.Inourexperienceabsorptionat280nmisextremelylowandhencewedonotcheckeitherthepurityorquantitateat280nm.TausDoyourunmassspecsforTausoronlygel?PurityassayfortauisdonebySDSonly,nomassspec.DoyourTauProteinshavehistags?OurTauproteinsdonothavehistags.AdvantageofTauproteinswithouthistagvstauproteinswithhistag:1.Tauproteinwithhis-tagismorelikelytobeinsolubleandhenceinamenableforvariousbiochemicalassays.ThisproblemwillnotbethereintheTauproteinwithoutanytag.2.Tauproteinwithhis-tagismorelikelytohavecontaminatingproteinswhichwillbecomingduetothenon-specificnatureofinteractionbetweenpolyhistidinetagsandNi2+ions.TheseproteinswillinterferewithanyoftheassaysorfunctionsofTauproteins.3.TherearechancesthatsomeNickeltoocancomeascontaminantwhichcanseverelyalterthefunctionsofTau.4.Ahis-tagTaucanalsobeimmunogenicifusedinhumans.Therecanbeimmuneresponseagainstthepolyhistidinetagwhichhasthepotentialtoactasalinearepitope.HowcanyourTausbeused?Ourproductsarenotforhumanuse,theyareforresearchpurposesonly.HowshouldyourlyophilizedTaubestored?ThelyophilizedTaucanbestoredforatleast2yearsat-80°C.HowshouldyourTaubestoredafterreconstitutioninwater?Afterreconstitutioninwater,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.Forlongerterm,itsbesttolyophilizeit.WhatisthestabilityoflyophilizedTaus?ThelyophilizedTauisstableforatleast2yearsat-80°C.Afterreconstitutioninwater,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.Forlongerterm,itsbesttolyophilizeit.WhichoneoftheTauproteinisoformsthatyousaleisthelongestorwhichonesarethemostphosphorylated?Tau441isthebiggesttauthatwehave.SincetheyaremadeinE.colitheyarenotphosphorylated.SynucleinsHowcanyourSynucleinsbeused?Ourproductsarenotforhumanuse,theyareforresearchpurposesonly.HowshouldyourlyophilizedSynucleinbestored?ThelyophilizedSynucleincanbestoredforatleast2yearsat-80°C.HowshouldyourSynucleinbestoredafterreconstitutioninwater?Afterreconstitutioninwater,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.Forlongerterm,itsbesttolyophilizeit.IwonderwhetherduringtheE.coliexpressionandproteinpurificationofyoursynucleinproducts,acompoundcalled"trifluoroacetate",isinvolvedinanyofthesteps?Trifluoroacetateisnotusedduringanyofthestepsinthesynucleinpurification.RegardingAlphaSynuclein:Istheproteinfulllength?Isittagged,ifso,withwhat?TheAlphaSynucleinisfulllengthandhasnotag.WhatisthestabilityoflyophilizedSynucleins?ThelyophilizedSynucleinisstableforatleast2yearsat-80°C.Afterreconstitutioninwater,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.Forlongerterm,itsbesttolyophilizeit.AntibodiesHowcanyourantibodiesbeused?Ourproductsarenotforhumanuse,theyareforresearchpurposesonly.OtherHowcanyourproductsbeused?Ourproductsarenotforhumanuse,theyareforresearchpurposesonly.BetaAmyloidsStabilityHowshouldyourBetaAmyloidbestoredafterreconstitutioninammoniumhydroxide?Afterreconstitutioninammoniumhydroxide,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.orlongerterm,itsbesttolyophilizeit.HowshouldyourlyophilizedBetaAmyloidbestored?ThelyophilizedBetaAmyloidcanbestoredforatleast2yearsat-80°C.WhatisthestabilityofyourlyophilizedBetaAmyloid?ThelyophilizedBetaAmyloidisstableforatleast2yearsat-80°C.Afterreconstitutioninammoniumhydroxide,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.orlongerterm,itsbesttolyophilizeit.TausStabilityHowshouldyourlyophilizedTaubestored?ThelyophilizedTaucanbestoredforatleast2yearsat-80°C.HowshouldyourTaubestoredafterreconstitutioninwater?Afterreconstitutioninwater,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.Forlongerterm,itsbesttolyophilizeit.WhatisthestabilityoflyophilizedTaus?ThelyophilizedTauisstableforatleast2yearsat-80°C.Afterreconstitutioninwater,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.Forlongerterm,itsbesttolyophilizeit.SynucleinsStabilityHowshouldyourlyophilizedSynucleinbestored?ThelyophilizedSynucleincanbestoredforatleast2yearsat-80°C.HowshouldyourSynucleinsbestoredafterreconstitutioninwater?Afterreconstitutioninwater,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.Forlongerterm,itsbesttolyophilizeit.WhatisthestabilityoflyophilizedSynucleins?ThelyophilizedSynucleinisstableforatleast2yearsat-80°C.Afterreconstitutioninwater,itsbesttoaliquotit(AVOIDFREEZETHAW)andstoreat-80°C.Thisshouldbestableforabout1-2weeks.Forlongerterm,itsbesttolyophilizeit.
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