JPT/Phosphorylation SiteDetector NKTK/PD072/

PhosphorylationSite-DetectorNKTK

ProductCode:PD-072

Peptidemicroarraydisplaying208peptidesderivedfromapeptidescan(15merswith8aaoverlap)throughNKTK(Swiss-ProtID:P30414)ofHomosapiens(Human).
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededforincubation)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Phosphositedetection,Substrateidentification
Indication(s)/Topic(s):Kinase
DeliveryTime:10-12days

PhosphorylationSite-Detectors
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesenablingcharacterizationofenzymessuchaskinasesaswellasmethyl,glycosyl,acetyltransferases.Peptidesareimmobilizedonglassslidesviaaflexiblelinker.Chemoselectivecouplinggeneratesmicroarraysdisplayingcovalentlyattachedpeptidesinfouridenticalsubarrays(3copiesofpeptidewithineachsubarray).Incubationcanbeperformedmanuallyorautomated.

BenefitsofJPTsPhosphorylationSite-Detector
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourenzymepreparationisneededforincubation
-Applicabletoavarietyofbiologicalsamples(e.g.serum,lysate,kinase)
-Lowbackgroundonglasssurface
-Read-outusingfluorescenctlylabeledantibodiesorradioisotopicallylabeledATP
-Screenthousandsofpotentialpeptidesubstrateseconomically

PhosphorylationSite-DetectorNKTK

SelectedReferences
"Parvulin17-CatalyzedTubulinPolymerizationisRegulatedbyCalmodulininaCalcium-DependentManner"
Burgardtetal,JBiolChem.(2015)-PMID:25940090
"PhosphopeptideDetectionwithBiotin-LabeledPhos-tag"
Kinoshita-Kikutaetal.,MethodsinMolecularBiology (2016)-PMID:26584916
"PKc-thetaisaNovelsc35SplicingFactorRegulatorinResponsetoTcellActivation"
McCuaigetal.,FrontiersinImmunology -PMID: n.a.
"Phos-tagTechnologyforKinomics"
Kinoshita-Kikutaetal.,Kinomics:ApproachesandApplications(2015)-PMID:n.a.
"CharacterizationoftheHostResponsetoPichindeVirusInfectionintheSyrianGoldenHamsterbySpecies-SpecificKinomeAnalysis"
Falcinellietal.,Mol.Cell.Proteomics.(2015)-PMID:25573744
"AntiviralPotentialofERK/MAPKandPI3K/AKT/mTORSignalingModulationforMiddleEastRespiratorySyndromeCoronavirusInfectionasIdentifiedbyTemporalKinomeAnalysis"
Kindrachuketal.,AntimicrobAgentsChemother.(2015)-PMID:25487801

ApplicationNotesforEnzymeSubstrateMicroarrays
"Exploringthehumanacetylomeusinghigh-densitypeptidemicroarrays"
Maschetal.,ApplicationNote(2012)(fulltext)
"UnravelingPhosphorylationoftheSplicingFactorSC35bytheDualSignalingKinasePKCinHumanT-Cells"
Raoetal.,ApplicationNote(2016)(fulltext)
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistoneModifyingEnzymeComplex"
Wilczeketal.,ApplicationNote(2013)(fulltext)

Testimonial
"WithJPT´speptidemicroarrayplatform,high-throughputkinaseprofilingusingsmallamountsofcelllysatebecomesfeasible,providingaccesstothediscoveryandmonitoringofnovelbioMarkersinParkinsonsDisease."
JeremyNichols,ParkinsonssInstitute,Sunnyvale,USA