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Bpsbioscience/BRD3 (BD2), GSTtag/31033/100 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/BRD3 (BD2), GSTtag/31033/100 µg
Species:Human
HostSpecies:E.coli
MW:40kDa
Genbank#:NM_007371
Tag(s):N-terminalGST-tag
a.a:306-417
Description:
HumanBromodomaincontaining3,alsoknownasBRD3orRING3L,GenBankAccessionNo.NM_007371,a.a.306–417correspondingtobromodomain2withN-terminalGST-tag,MW=40kDa,expressedinanE.coliexpressionsystem
UniProt
Q15059
Synonym(s):
Bromodomaincontaining3,BRD3,RING3L
AssayConditions:

EnzymewasassayedaccordingtoBPSBRD3(BD2)AssayKit(Cat#32523)protocol.BRD3proteinisincubatedwithbiotinylatedacetylhistonepeptidesubstrate(Cat.#33000)inBRDassaybuffer(Cat.#33001)ina10µlreactionfor1houratRT.AlphaLISA®(R)GSHacceptorbeads(Perkinelmer)areadded,followedbyAlphascreen®(R)Streptavidin-conjugateddonorbeads(PerkinElmer).After1hourincubationatRT,Alphacountsaremeasured.

Formulation:
50mMTris-HCl,pH8.0,138mMNaCl,2.7mMKCl,10%glycerol,3mMEDTA,20mMglutathione,and2mMDTT
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofbromodomainbindingassays,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Gamsjaeger,R.,etal.,MolCellBiol.2011Jul;31(13):2632-2640.
2.Lamonica,JM,etal.,ProcNatlAcadSciUSA.2011May31;108(22):E159-168.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Bromodomain
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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