Agrisera/Rnr1 | Ribonucleosidediphosphate reductase large subunit/AS16 3639/


productinformation
Background		SaccharomycescerevisiaeRnr1(EC=1.17.4.1)isanenzymefromribonucleosidediphosphatereductase largechainfamily.IslocalizedtocytoplasmandprovidesprecursorsnecessaryforDNAsynthesis.Alternativenames:ribonucleotidereductaselargesubunit1,ribonucleotidereductaseR1subunit1
Immunogen		KLH-conjugatedsyntheticpeptidederivedfromSaccharomycescerevisiaeRnr1proteinsequenceP21524
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50µl
Reconstitution		Forreconstitutionadd50µlofsterilewater.
Storage		Storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.
Testedapplications		Westernblot(WB)
Relatedproducts		AS09574\|anti-Rnr3antibodies AS09575\|anti-Rnr2antibodies AS10847\|anti-Sml1\|SuppressorofMec1lethality Secondaryantibodies
Additionalinformation

applicationinformation
Recommendeddilution		1:5 000-1:10000withECL(WB)
Expected\|apparentMW		99.56\|100kDa
Confirmedreactivity		Saccharomycescerevisiae
Predictedreactivity		Saccharomycescerevisiae
Notreactivein		Noconfirmedexceptionsfrompredictedreactivityknowninthemoment.
Additionalinformation
Selectedreferences		Tobeaddedwhenavailable,antibodyreleasedinMarch2016.

applicationexample

10uloftotalproteinfroma1x108cells/mlcultureofSaccharomycescerevisiaeextractedwith20%TCAandre-suspendedin1Xloadingbuffer(4XSDSloadingbuffer:150mMTrisHClpH7.0,25%Glycerol,12%SDS,0.02%BromophenolBlue,5%B-mercaptoethanol)wereseparatedon7.5%SDS-PAGEandblottedtonitrocellulosemembranefor2h/60Vusingtanktransfer.Blotswereblocked(5%milk)for1hatroomtemperature(RT)withagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:10000or1:5000o/nat4°C.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflytwice,thenwashed3timesfor10mininTBS-TatRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGhorseradishperoxidaseconjugated,AS09602)dilutedto1:50000or1:75000in5%milkfor1hatRTwithagitation.TheblotwaswashedasaboveanddevelopedwithWesternLightningECLPro(PerkinElmer).Exposuretimewasbetween30secondsand1minutedependingonthedilutions.

CourtesyofDr.IsaacCorcoles,NWCR,UnitedKingdom

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Agrisera/Rnr1 | Ribonucleosidediphosphate reductase large subunit/AS16 3639/