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Innovative Research/Mouse Hemopexin ELISA Kit/IRKTAH1153/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Innovative Research/Mouse Hemopexin ELISA Kit/IRKTAH1153/1 Ea

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MouseHemopexinELISAKit

InthisassaytheHemopexinpresentinsamplesreactswiththeanti-hemopexinantibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundproteinsbywashing,anti-HXantibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundHX.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3,5,5-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofHXinthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofHXinthetestsample.ThequantityofHXinthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedfor
sampledilution.

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