产品资料

ProteinTech/FUS/TLS Monoclonal antibody/150ul/60160-1-Ig

Tested Applications

Positive IP detected in	HeLa cells
Positive IHC detected in	human gliomas tissue, human ovary tumor tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	human brain(ALS) tissue
Positive FC detected in	K-562 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Application	Dilution
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)	IHC : 1:500-1:2500
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

60160-1-Ig targets FUS/TLS inWB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	Drosophila, human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	FUS/TLS fusion protein Ag2150
Full Name	fusion (involved in t(12;16) in malignant liposarcoma)
Calculated molecular weight	75 kDa
Observed molecular weight	75 kDa
GenBank accession number	BC026062
Gene symbol	FUS
Gene ID (NCBI)	2521
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. Two research groups simultaneously reported that FUS is present in 5% of the pathalogical aggregations (inclusions) seen in familial amyotrophic sclerosis (fALS). FUS-positive inclusions were also reported in cases of sporadic ALS (sALS). More recently, wild-type FUS has also been implicated in the pathological development of frototemporal lobar dementia (FTLD) with ubiquitin-positive inclusions (FTLD-U), further linking FUS to the pathogenesis of neurogenerative diseases. There is some debate as to whether FUS colocalizes with TDP-43 in TDP-43-positive cases of ALS and whether TDP-43 and FUS cause neurodegenerative disease independently or contributively of one another. This antibody is a mouse monoclonal antibody raised against an internal region of human FUS. Initial reports from our customers suggest this new monoclonal FUS antibody (60160-1-Ig) is a useful tool in ALS and FTLD research. For more details, please see our blog article regarding the matter.

Protocols

Product Specific Protocols
WB protocol for FUS/TLS antibody 60160-1-Ig	Download protocol
IHC protocol for FUS/TLS antibody 60160-1-Ig	Download protocol
IP protocol for FUS/TLS antibody 60160-1-Ig	Download protocol
FC protocol for FUS/TLS antibody 60160-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols