产品资料

ProteinTech/TDP-43 Polyclonal antibody/150ul/10782-2-AP

Tested Applications

Positive WB detected in	SH-SY5Y cells, HeLa cells,K-562 cells,C2C12 cells,Neuro-2a cells
Positive IHC detected in	mouse brain tissue, human brain tissue,human brain (FTLD-U) tissue,human gliomas tissue,human pancreas tissue,rat brain tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HepG2 cells, HeLa cells,SH-SY5Y cells
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 15 publications below
WB	See 464 publications below
IHC	See 555 publications below
IF	See 308 publications below
IP	See 32 publications below
ELISA	See 3 publications below
CoIP	See 4 publications below
ChIP	See 4 publications below
RIP	See 2 publications below

Product Information

10782-2-AP targets TDP-43 inWB, RIP, IP, IHC, IF, IEM, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity	human, mouse, rat, zebrafish
Cited Reactivity	C. elegans, chicken, dog, Drosophila, fly, hamster, horse, human, monkey, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	fusion protein
Full Name	TAR DNA binding protein
Calculated molecular weight	43 kDa
Observed molecular weight	43 kDa
GenBank accession number	BC001487
Gene symbol	TARDBP
Gene ID (NCBI)	23435
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech"s 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications.Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Protocols

Product Specific Protocols
WB protocol for TDP-43 antibody 10782-2-AP	Download protocol
IHC protocol for TDP-43 antibody 10782-2-AP	Download protocol
IF protocol for TDP-43 antibody 10782-2-AP	Download protocol
FC protocol for TDP-43 antibody 10782-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols