产品资料

ProteinTech/Caspase 3/p17/p19 Polyclonal antibody/150ul/19677-1-AP

Tested Applications

Positive WB detected in	Jurkat cells, mouse spleen tissue,HeLa cells,rat brain tissue,rat liver tissue
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	mouse brain tissue, human teeth tissue,human spleen tissue,human kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	NIH/3T3 cells, mouse brain tissue,HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 708 publications below
IHC	See 64 publications below
IF	See 21 publications below
ELISA	See 1 publications below

Product Information

19677-1-AP targets Caspase 3/p17/p19 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	Astragalus membranaceus, Bovine, chicken, cow, duck, goat, Hamster, hamsters, human, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Full Name	caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight	32 kDa
Observed molecular weight	32-35 kDa, 17 kDa, 19 kDa
GenBank accession number	NM_004346
Gene symbol	CASP3
Gene ID (NCBI)	836
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Protocols

Product Specific Protocols
WB protocol for Caspase 3/p17/p19 antibody 19677-1-AP	Download protocol
IHC protocol for Caspase 3/p17/p19 antibody 19677-1-AP	Download protocol
IF protocol for Caspase 3/p17/p19 antibody 19677-1-AP	Download protocol
IP protocol for Caspase 3/p17/p19 antibody 19677-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols