产品资料

ProteinTech/Caspase 8/p43/p18 Polyclonal antibody/150ul/13423-1-AP

Tested Applications

Positive WB detected in	Staurosporine treated Jurkat cells, RAW264.7,HeLa cells,Raji cells,Jurkat cells,UV treated Hela,Sp2/0 cells
Positive IP detected in	HeLa cells, Jurkat cells
Positive IHC detected in	human lymphoma tissue,human colon tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 128 publications below
IHC	See 8 publications below
IF	See 2 publications below
ELISA	See 1 publications below

Product Information

13423-1-AP targets Caspase 8/p43/p18 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human,mouse samples.

Tested Reactivity	human,mouse
Cited Reactivity	chicken, human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Caspase 8/p43/p18 fusion protein Ag4214
Full Name	caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight	538 aa, 62 kDa
Observed molecular weight	53-57 kDa, 32-45 kDa
GenBank accession number	BC028223
Gene symbol	CASP8
Gene ID (NCBI)	841
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Protocols

Product Specific Protocols
WB protocol for Caspase 8/p43/p18 antibody 13423-1-AP	Download protocol
IHC protocol for Caspase 8/p43/p18 antibody 13423-1-AP	Download protocol
IF protocol for Caspase 8/p43/p18 antibody 13423-1-AP	Download protocol
IP protocol for Caspase 8/p43/p18 antibody 13423-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols