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| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HEK-293 cells |
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
| WB | See 1 publications below |
16617-1-AP targets CAD inWB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CAD fusion protein Ag9912 |
| Full Name | carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase |
| Calculated molecular weight | 2225 aa, 243 kDa |
| Observed molecular weight | 240 kDa |
| GenBank accession number | BC014178 |
| Gene symbol | CAD |
| Gene ID (NCBI) | 790 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
| Product Specific Protocols | |
|---|---|
| WB protocol for CAD antibody 16617-1-AP | Download protocol |
| IHC protocol for CAD antibody 16617-1-AP | Download protocol |
| IF protocol for CAD antibody 16617-1-AP | Download protocol |
| IP protocol for CAD antibody 16617-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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