Positive WB detected in | human stomach tissue,HL-60 cells,mouse small intestine tissue |
Positive IHC detected in | human stomach cancer tissue,human kidney tissue,human lung tissue,human ovary tissue,human stomach tissue,human testis tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
WB | See 1 publications below |
IHC | See 1 publications below |
17556-1-AP targets Calpain 9 inWB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Calpain 9 fusion protein Ag11718 |
Full Name | calpain 9 |
Calculated molecular weight | 627 aa, 72 kDa |
Observed molecular weight | 60-66 kDa |
GenBank accession number | BC027993 |
Gene symbol | CAPN9 |
Gene ID (NCBI) | 10753 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).
Product Specific Protocols | |
---|---|
WB protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
IHC protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
IF protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn