Deprecated: Creation of dynamic property cls_session::$session_data_table is deprecated in /www/sites/www.188bio.com/index/systems/cls_session.php on line 49
ProteinTech/Caspase 10/p23/17/p12 Polyclonal antibody/150ul/14311-1-AP188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线
产品资料

ProteinTech/Caspase 10/p23/17/p12 Polyclonal antibody/150ul/14311-1-AP

Tested Applications

Positive WB detected inHeLa cells,Apoptosised
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14311-1-AP targets Caspase 10/p23/17/p12 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivityhuman
Host / IsotypeRabbit / IgG
ClassPolyclonal
TypeAntibody
ImmunogenCaspase 10/p23/17/p12 fusion protein Ag5511
Full Namecaspase 10, apoptosis-related cysteine peptidase
Calculated molecular weight 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC042844
Gene symbolCASP10
Gene ID (NCBI)843
ConjugateUnconjugated
FormLiquid
Purification MethodAntigen affinity purification
Storage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.

Background Information

Caspase10(CASP10)isalsocalledMCH4,isamemberofthepeptidaseC14Afamily,andcontainstwodeath

effector(DED)domains.

WhatisthemolecularweightofCASP10?

ThemolecularweightofCASP10observedonWesternblotsmayvarydependingonwhichofitsisoform,

truncation,orcleavageformsarepresent.Full-lengthCASP10hasapropeptidewith219aminoacidand

6isoformsproducedbyalternativesplicing.

WhatisthetissuespecificityofCASP10?

CASP10isdetectableinmosttissues.However,thereislowerexpressionobservedinbrain,kidney,colon,

prostate,andtestistissue.

WhatisthefunctionofCASP10?

CASP10playsaroleinthecascadeofcaspasesresponsibleforexecutingapoptosis.Itisrecruitedtoboththe

FasandTNFR-1receptorsinaFADD-dependentmanner.CASP10isresponsibleforthecleavageandactivationof

caspases3,4,6,7,8,and9.CASP10canbecleavedbygranzymeBandautocatalyticactivitytogeneratetwo

activesubunitsfromitsinactiveproenzymeform.

WhatistheroleofCASP10indisease?

DefectsinCASP10leadtoautoimmunelymphoproliferativesyndrometype2A(ALPS2A),whichischaracterized

byabnormallymphocyteanddendriticcellhomeostasis,aswellasimmuneregulatorydefects(PMID:16446975).

DefectsinCASP10arealsoacauseoffamilialnon-Hodgkinlymphoma(NHL)andoftenmarkedbyenlargedlymph

nodes,weightlossandfever(PMID:12010812),andgastriccancers(GASC)(PMID:11973654).

Protocols

Product Specific Protocols
WB protocol for Caspase 10/p23/17/p12 antibody 14311-1-APDownload protocol
IHC protocol for Caspase 10/p23/17/p12 antibody 14311-1-APDownload protocol
IF protocol for Caspase 10/p23/17/p12 antibody 14311-1-APDownload protocol
IP protocol for Caspase 10/p23/17/p12 antibody 14311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
新闻动态
行业前沿
技术文章
最新产品