Positive WB detected in | HeLa cells,Apoptosised |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human endometrial cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
14311-1-AP targets Caspase 10/p23/17/p12 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Caspase 10/p23/17/p12 fusion protein Ag5511 |
Full Name | caspase 10, apoptosis-related cysteine peptidase |
Calculated molecular weight | 59 kDa |
Observed molecular weight | 59 kDa |
GenBank accession number | BC042844 |
Gene symbol | CASP10 |
Gene ID (NCBI) | 843 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Caspase10(CASP10)isalsocalledMCH4,isamemberofthepeptidaseC14Afamily,andcontainstwodeath
effector(DED)domains.
WhatisthemolecularweightofCASP10?
ThemolecularweightofCASP10observedonWesternblotsmayvarydependingonwhichofitsisoform,
truncation,orcleavageformsarepresent.Full-lengthCASP10hasapropeptidewith219aminoacidand
6isoformsproducedbyalternativesplicing.
WhatisthetissuespecificityofCASP10?
CASP10isdetectableinmosttissues.However,thereislowerexpressionobservedinbrain,kidney,colon,
prostate,andtestistissue.
WhatisthefunctionofCASP10?
CASP10playsaroleinthecascadeofcaspasesresponsibleforexecutingapoptosis.Itisrecruitedtoboththe
FasandTNFR-1receptorsinaFADD-dependentmanner.CASP10isresponsibleforthecleavageandactivationof
caspases3,4,6,7,8,and9.CASP10canbecleavedbygranzymeBandautocatalyticactivitytogeneratetwo
activesubunitsfromitsinactiveproenzymeform.
WhatistheroleofCASP10indisease?
DefectsinCASP10leadtoautoimmunelymphoproliferativesyndrometype2A(ALPS2A),whichischaracterized
byabnormallymphocyteanddendriticcellhomeostasis,aswellasimmuneregulatorydefects(PMID:16446975).
DefectsinCASP10arealsoacauseoffamilialnon-Hodgkinlymphoma(NHL)andoftenmarkedbyenlargedlymph
nodes,weightlossandfever(PMID:12010812),andgastriccancers(GASC)(PMID:11973654).
Product Specific Protocols | |
---|---|
WB protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP | Download protocol |
IHC protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP | Download protocol |
IF protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP | Download protocol |
IP protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn