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ProteinTech/CDK2 Monoclonal antibody/150ul/60312-1-Ig

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue,human cervical cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below
IHCSee 1 publications below

Product Information

60312-1-Ig targets CDK2 inWB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivityhuman
Cited Reactivity human, mouse
Host / IsotypeMouse / IgM
ClassMonoclonal
TypeAntibody
ImmunogenCDK2 fusion protein Ag21310
Full Namecyclin-dependent kinase 2
Calculated molecular weight 33 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC003065
Gene symbolCDK2
Gene ID (NCBI)1017
ConjugateUnconjugated
FormLiquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CDK2 antibody 60312-1-IgDownload protocol
IHC protocol for CDK2 antibody 60312-1-IgDownload protocol
FC protocol for CDK2 antibody 60312-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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