产品资料

Rubicon Genomics/ThruPLEX DNA-seq 48S Kit (48 reactions, 48 single indexes)/R400427/1 Ea

ThruPLEXDNA-seqKitOutperformsOtherNGSLIbraryPrepKits

ThruPLEXDNA-seqKityieldedmorediverselibraries(left)withlessinputDNAandgeneratedfewerduplicates(right)at25Mreadpairsthanotherkits.NGSlibrarieswerepreparedwiththeindicatedamountofreferencegDNAusingthelibrarypreparationkitsindicatedandsequencedonaMiSeq,v3flowcell.

ThruPLEXDNA-seqKitSingle-TubeWorkflow

Startingwithaslittleas50pgoffragmenteddouble-strandedDNAorCDNA,ThruPLEXDNA-seqKitcreatesindexedlibrariesin3simplesteps:endrepair,adapterligation,andhigh-fidelitylibraryamplification.Thestreamlinedworkflowpreventssamplelossandisperformedinabout2hoursinasingletubeorwellpreventingsamplelossandenhancingpositivesampleidentification.

ThruPLEXDNA-seqKitMinimizesGCBias

Relativecoverageofthehumangenomeasafunctionof%GC(50nginputDNA).(Left)ThruPLEXDNA-seqKithasexcellentcoveragethroughouttheentirerange.(Right)ThruPLEXDNA-seqKit(gold)performsaswellasorbetterthanotherkitstested.Notethattheoptimalcoverageshouldbeequalto1inthesegraphs.GCcompositiondistributionofthehumangenomeisrepresentedbytheredbars.

ThruPLEXDNA-seqKitHasaLowerandBroaderInputRange

ThruPLEXDNA-seqKithasalowerandbroaderrecommendedinputrangethanotherkits.ThesampleinputamountforThruPLEXDNA-seqKitis20timesmoresensitivethanotherkits,rangingfrom50pgand50ng andrequiresnooptimizationoftheprotocoloradapterconcentration. Indexesandadaptersareincludedwiththekit.

ThruPLEXDNA-seqKitWorkflowAdvantage:HighQualityNGSLibrariesinAbout2Hours

ThruPLEXDNA-seqKitconvertsdouble-strandedDNAsamplestoindexedlibrariesin3simplesteps:endrepair,adapterligation,andhigh-fidelitylibraryamplification.Thestreamlinedworkflowpreventssamplelossandhandlingerrorsandisperformedinasingletubeorwellinlessthan2hours.

ThruPLEXDNA-seqKitTechnology

ThruPLEXtechnologyisa3-stepreactionthatstartswithfragmenteddouble-strandedDNA(0.05ngto50ng)whichisrepairedinahighlyefficientprocess.Backgroundisreducedusingdouble-strandedadaptorswithnosingle-strandedtails.Bluntendligationoccurswithhigh-efficiency.Blocked5’endsreduceadaptor-adaptorligation.Backgroundisfurtherreducedbydestroyingunusedadaptorsafterligation

Storeat-20°C.

Guaranteedfor9 monthsat-20°Cinaconstanttemperaturefreezer.

CAT.NO.R400406ThruPLEXDNA-seq48DKit(48reactions,48dualindexes)

CAT.NO.R400407ThruPLEXDNA-seq96DKit(96reactions,96dualindexes)

CAT.NO.R400427ThruPLEXDNA-seq48SKit(48reactions,48singleindexes)

CAT.NO.R400428ThruPLEXDNA-seq12S(48)Kit(48reactions,12singleindexes)

CAT.NO.R400523ThruPLEXDNA-seq6S(12)Kit(12reactions,6singleindexes)

ThruPLEXDNA-seqcontainseverythingneeded,includingindexesinabarcoded96-wellplate.

Highperformancefromlowestinput:50pgto50ng

Completepackageforcreatinglibraries:optimizedindexingandadaptorreagents,enzymes,buffers,andnuclease-freewater

Fastandsimpleworkflow:3stepsinasingletubeorwellinabout2hours,notransfersnecessary

CompatIBLewithmajortargetenrichmentplatforms:AgilentSureSelect^®,RocheNimblegen^®SeqCap^®EZ,andIDTxGen^®Lockdown^®probes