The tumor suppressor role of Src homology phosphotyrosine phosphatase 2 in hepatocellular carcinomaC Jiang, F Hu, Y Tai, J Du, B Mao, Z Yuan… - Journal of Cancer …, 2012... incubated with 10% normal goat serum for 30 min, and then incubated with the primary antibody(Bioworld Technology, BS1705 ... Shp2 antibody (Bio- world Technology, BS1705, diluted 1:1,000)was used, and anti-human GAPDH antibody (CoWin Biotech, CW0266, diluted 1 ...Hypoxia-inducible Factor Activation in Myeloid Cells Contributes to the Development of Liver Fibrosis in Cholestatic MiceBL Copple, S Kaska… - Journal of Pharmacology and Experimental …, 2012... minutes. Sections of frozen liver were fixed in acetone for 10 minutes at -20°C. Sections wereincubated with rabbit polyclonal anti-PDGF-B antibody (Bioworld Technology, Inc., St. Louis Park,MN) diluted 1:50 in PBS containing 3% goat serum at room temperature for 3 hours. ...Impairment of cellular immunity is associated with overexpression of heat shock protein 70 in neonatal pigs with intrauterine growth retardationX Zhong, W Li, X Huang, L Zhang, M Yimamu… - Cell Stress and …, 2012... IκB kinase (IKK) assay The IKK activity was assessed using an in vitro kinase assay as previouslydescribed (Yoo et al. 2000). Rabbit polyclonal anti-IKKα/β antibody (Bioworld, Dublin, OH, USA)was used to immunoprecipitate the IKK complex for determina- tion of IKK activity. ...HnRNPL as a key factor in spermatogenesis: Lesson from functional proteomic studies of azoospermia patients with sertoli cell only syndromeJ Li, W Guo, F Li, J He, Q Yu, X Wu, J Li… - Journal of Proteomics, 2012... activated-caspase-3 proteins in the samples were probed by rabbit polyclonal primary antibodyagainst HnRNPL (diluted 1:500; Abcam, Cambridge, United Kingdom) and monoclonal primaryantibody against activated-caspase-3-p17 (diluted 1:500; Bioworld Technology, Louis ...[PDF] Leptin regulates proliferation and apoptosis of colorectal carcinoma through PI3K/Akt/mTOR signalling pathwayD Wang, J Chen, H Chen, Z Duan, Q Xu, M Wei… - J. Biosci, 2012... For ObR, we used B-3 mouse mAb, 1:200 (Santa Cruz); and for mTOR pAb;1:1000 (P2476,Bioworld Technology, Inc, USA) and p-mTOR pAb, 1:1000 (ser-2448; Bioworld Technology, Inc,USA), anti-β-actin antibody was used as a loading control. 2.11 Statistical analysis ...Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathwaysY Yu, J Mu, Z Fan, G Lei, M Yan, S Wang… - Histochemistry and Cell …, 2012... These cells were purified by using rabbit anti-STRO-1 antibody (Santa Cruz, Delaware, CA) andsheep anti-rabbit IgG Dynabeads (Dynal ... ERK1/2, 1:1,000, Bioworld; JNK1/ 2/3, 1:1,000, Bioworld;phosphor-JNK1/2/3, 1:1,000, Bio- world; P38, 1:1,000, Bioworld; phosphor-P38, 1 ...Reversal effect of Tween-20 on multidrug resistance in tumor cells in vitroS Yang, JJ Liu, Y Chen… - Biomedicine Pharmacotherapy, 2012... Ltd, Nanjing, China). P-gp antibody was from Santa Cruz Biotechnology (Santa Cruz, CA).P-Tubulin antibody was from Bioworld (Bioworld Technology, MN). All other chemicals wereof the highest commercial grade available. 2.2. Cell lines and cell culture. ...Inhibition of IGF‐IR increases chemosensitivity in human colorectal cancer cells through MRP‐2 promoter suppressionK Shen, D Cui, L Sun, M Han… - Journal of Cellular Biochemistry, 2012... California, USA). Antibodies against IGF-IR and phospho-IGF-IR-Tyr1161 were fromBioworld (
Bioworld Technology Co. Ltd., Minnesota, USA). P-gp antibody was fromeBioscience (eBioscience Inc., California, USA). Dual luciferase ...ROCK1 induces ERK nuclear translocation in PDGF‐BB‐stimulated migration of rat vascular smooth muscle cellsY Zhao, M Lv, HS Lin, Y Hong, FC Yang, YL Sun… - IUBMB life, 2012... membrane was incubated with rabbit ROCK1 polyclonal antibody (Abcam, Cambridge, MA, USA),rabbit ROCK2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit actinpolyclonal antibody (Cell Signaling Technology), ERK (Bioworld Technology, St. ...The prostate cancer-up-regulated long noncoding RNA PlncRNA-1 modulates apoptosis and proliferation through reciprocal regulation of androgen receptorZ Cui, S Ren, J Lu, F Wang, W Xu, Y Sun, M Wei… - … Oncology: Seminars and …, 2012... sc-816, 1:1000; Santa Cruz Biotechnology, Santa Cruz, CA), cleaved-PARP rabbit antibody(#9541S, 1:1000; Cell Signaling Technology, Inc., Beverly, MA) and incubated overnight at4°C and with internal control GAPDH antibody (#470661, 1:2000; Bioworld, Visalia, CA ... 美国进口bioworld
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