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Trevigen/Calcein AM Cell Viability Assay Kit/4892-010-K/1000 Wells

Description

CalceinAMisanon-fluorescent,hydrophobiccompoundthateasilypermeatesintact,livecells.ThehydrolysisofCalceinAMbyintracellularesterasesproducescalcein,ahydrophilic,stronglyfluorescentcompoundthatiswell-retainedinthecellcytoplasm.Cellsgrown,preferablyinblack-walledplates,canbestainedandquantifiedinlessthantwohours.

Features:

  • Suitableforproliferatingandnon-proliferatingcells.
  • IdealforbothsUSPensionandadherentcells.
  • Non-rADIoactiveassay.
  • Rapid(nosolubilizationstepasinaconventionalMTTassay).
  • Idealforhigh-throughputexperiments.
  • Betterretentionandbrightnesscomparedtootherfluorescentcompounds(e.g.fluorescein).
  • Usefulinavarietyofstudies,including:celladhesion,chemotaxis,multidrugresistance,cellviABIlity,apoptosis,andcytotoxicity.
  • Adaptabletoawidevarietyoftechniques,including:microplateassays,immunocytochemistry,flowcytometry,andinvivocelltracking.

 

Catalog#4892-010-Kincludes:
CatalogNumberDescriptionQty
4892-010-01CalceinAM2
4892-010-0210XCalceinAMDWBuffer1

Prolyl-4-hydroxylasedomain3(PHD3)isacriticalterminatorforcellsurvivalofmacrophagesunderstressconditions
LijaSwain,MariekeWottawa,AnnetteHillemann,AngelikaBeneke,HarukiOdagiri,KazutoyoTerada,MotoyoshiEndo,YuichiOike,KatjaFarhat,andDörtheM.Katschinski
J.Leukoc.Biol.,Sep2014;96:365–375.
http://www.jleukbio.org/cgi/content/abstract/96/3/365

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