IsolationfromHEK293Cells:PARP1andTnks1
●RNF146WWEAffinityResinSetTheTulipBiolabs,Inc.RNF146WWEAffinityResinSetisdesignedfortheisolationofpoly-ADP-ribosylatedproteinsfromculturedcellsandtissuesamples.ThroughtheuseofahighlyspecificWWEaffinityresin,poly-ADP-ribosylated(PARylated)proteinsareisolatedfromcellortissuelysateswithouttheuseofanti-PARantibodies. Theresinboundproteinscanbeelutedfromtheaffinityresin,andanalyzedbyimmunoblottingorothermethods.
Cat.#4306Setcontains1eachofthefollowing(canbeorderedseparately):
•WWEResin,0.5mgWWEfusionproteinsuppliedasaslurrycontaining ~50µLresin,Cat.#2334
•WWENegControlResin,0.5mgfusionproteinsuppliedasaslurrycontaining~50µLresin, Cat.#2335
TheWWEAffinityResin,Cat.#2334ishighlypurifiedGST-RNF146(100-175)fusionproteinconstructexpressedinE.coli,andboundtoglutathionebeads.
WWENegativeControlResin,Cat.#2335isidenticaltothe#2334resinexceptforR163Asubstitution,whicheffectivelyabolishesPARbinding. Thenegativecontrolresinisusefultocontrolfornon-specificbinding,anditsuseisoptional.
Pleaseseethe Cat.#4306 Datasheet(pdf) foracompletedescription.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn