产品资料

TulipBiolabs//5 x 50 ug/2090-5 x 50 ug

InhibitionofPARP1by3-aminobenzamide

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AutomodificationofPARP1
SDS-PAGE/Coomassieblue

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AutomodificationofPARP1WBusinganti-PARP1

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PARP1Enzymehuman, highlyactivePoly(ADP-ribose)polymerase-1(PARP1)isanabundantandubiquitousnuclearenzymethatcatalyzestheNAD(+)-dependentadditionofADP-ribosepolymersonavarietyofnuclearproteins. TheTulipBiolabs,Inc. PARP1Enzyme,Cat.#2090,ishighlypurifiedhumanPARP1,expressedinabaculovirusexpressionsystem.ThePARP1hasahighspecificactivity,approx.20000U/mg(1U=10fmolADP-riboseincorporatedintoimmobilizedhistonein30min@RT). Itislyophilizedfromitsnativestateunderspecialconditions,anduponreconstitutionwithwater,itretainsitspolyADP-ribosylationenzymaticactivity. PuritybySDS-PAGEis>95%. Eachvialcontains50µgPARP1(approx.1000Units).

●Useinhigh-throughputenzymeactivity/inhibitionassays

●Poly-ADP-ribosylationoftargetproteins

●WesternBlot,100ng/laneusingHRP/TMBcolorimetricmethods

ELISA,2ng/well

●SDS-PAGE,1µg/lane●Asastandardinotherassays

Pleaseseethe Cat.#2090Datasheet(pdf) foracompletedescription. 

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