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NeoBiotechnologies/Bcl-2 (Apoptosis & Follicular Lymphoma Marker)/596-MSM9-P1

ProductDetails

Synonyms

ApoptosisregulatorBcl-2,B-cellCLL/lymphoma-2

PositiveControl

A431,U937,Jurkat,K562,HL-60orHeLacells.Tonsilorfollicularlymphomas.

KnownApplications&SuggestedDilutions

FlowCyt,IF,WesternBlot,IHC-PMoreDetails

FlowCytometry(1-2ug/millioncells)Immunofluorescence(1-2ug/ml)WesternBlot(1-2ug/ml)Immunohistochemistry(Formalin-fixed)(1-2ug/mlfor30minutesatRT)(Stainingofformalin-fixedtissuesisenhancedbyheatingtissuesectionsin10mMTriswith1mMEDTABuffer,pH9.0,for45minat95°CfollowedbycoolingatRTfor20minutes)Optimaldilutionforaspecificapplicationshouldbedetermined.

Immunogen

Recombinantfull-lengthhumanbcl-2protein

CellularLocalization

Outermitochondrialmembranesandendoplasmicreticulumaswellasnuclearmembranes.

SpeciesReactivity

Human.

Host/IgIsotype

Mouse/IgG1,kappa

Mol.WeightofAntigen

25-26kDa
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