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NeoBiotechnologies/Bcl-2 (Apoptosis & Follicular Lymphoma Marker)/596-MSM11-P0

Product Details

Synonyms

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

Positive Control

Jurkat, K562, HL-60 or HeLa cells. Tonsil or follicular lymphomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)Immunofluorescence (1-2ug/ml)Western Blot (1-2ug/ml)Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human BCL2 protein

Cellular Localization

Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

25-26kDa
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