SBI/miRZip™ Pooled Anti-miRNA Virus Library/MZIPPLVA-1-

HowItWorks

ScreeningwiththemiRZipLibraryisstraightforward

• TransducethemiRZipLibraryintotargetcells
• Treattoinducephenotype
• Useafunctionalassay(invasionassay,differentiationassay,proliferationassay,apoptosisassay,etc.)toselectforcellsexhibitingthedesiredphenotype
• IdentifymiReffectorsbyamplifyingeffectormiRandsequencing

SupportingData

SeeSBI’smirZiptechnologyinaction

miRZipconstructsexpresshighlevelsofanti-microRNAs

Figure1.miRZipconstructsexpresshighlevelsofanti-microRNAs.miRZipconstructsweretransfectedintoHEK293cells,totalRNAisolated,andmiRZiplevelsmeasuredusingSBI’sQuantiMirAssay(Cat.#RA420A-1).ComparedtoQuantiMirassaysperformedonRNAisolatedfromnon-transfectedcells,themiRZip-transfectedcellsdeliveredhighexpressionlevelsofthespecificmiRZipanti-miRNAs.

EfficientmodulationoftargetproteinlevelsbySBI’sLenti-miRandmiRZiptechnologies

Figure2.TargetproteinlevelsareefficientlymodulatedbySBI’sLenti-miR-29aandmiRZip-29amicroRNAconstructs.

miRZiptechnologyiseffectiveinafunctionalassay

Figure3.miRZip-21andmiRZip-145exhibitanti-oncogenicandoncogenicactivity,respectively,inacellinvasionassayusingMDA-MB-231breastcancercells.(Toppanel)miR-21isanoncogenicmiRNAandmiR-145isatumorsuppressingmiRNA.TransductionofmiRZip-21(theanti-miR21lentiviralconstruct)andmiRZip-145(theanti-miR145lentiviralconstruct),inhibitedtherespectivemiRs,resultinginalowerpercentageofinvADIngcellsinmiRZip-21-transducedcellsandahigherpercentageofinvadingcellsinmiRZip-145-transducedcells.(Bottompanel)Additionally,themiRZip-145lentivirusinhibitedendogenousmiR-145andelevatedproteinexpressionlevelsofthemiR-145targetoncogenec-Myc.

Resources

Citations

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