Supporting your studies with ready-to-go reference assays
Get full confidence in your QuantiMir-supported qPCR miRNA profiling studies with this kit for measuring endogenous miR-16 in human or mouse cells. Uses the same QuantiMir workflow and reagents as our QuantiMir Kit.
SBI offers the following reference assays:[table “quantimir-reference-assaysdocx” not found /]
The QuantiMir Kit enables robust miRNA quantitation through a simple and efficient workflow
Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.
The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.
Create custom miRNA assays
To design your own miRNA assays, simply synthesize an oligo using the sequence of the mature miRNA you’d like to profile as the forward primer in your miRNA qPCR assay, and use with the universal reverse primer included in the QuantiMir Kit.
Converting miRNA into cDNA for accurate qPCR profiling and quantitation
The QuantiMir Kit is sensitive
The QuantiMir Kit is accurate
Use the QuantiMir Kit to profile miRNAs from cancerous and normal tissues
Measure both siRNA and mRNA knockdown in a single QuantiMir cDNA sample
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn