EXOCET96A1,SBI,exosome quantitation,SBI,System Biosciences,systembio,+,外显子定量,Array.Array.Array蚂蚁淘厂家直采.

exosomequantitationSBI/EXOCETExosomeQuantitationKit/EXOCET96A-1/96Reactions
SystemBiosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创蚂蚁淘技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。现阶段研发重心为RNA干扰（RNAi）研究之相关工具。SystemBiosciences(SBI)致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI是专业的慢病毒产品公司，提供基于慢病毒的所有相关产�
Overview:

MovequicklywithSBI’sfastestexosomequantitationmethod

WiththeEXOCETExosomeQuantitationKit,youcanfindoutjusthowmanyexosomesyou’veisolatedinaslittleas20minutes.Thisantibody-free,colorimetricassay(OD405)measurestheactivityofAcetyl-CoAAcetylcholinesterase(AChE),anenzymeshowntobehighlyenrichedinexosomes1,2(usingmassspectrometryandWesternblotting,SBIhasverifiedthepresenceofAChEinexosomesfromserum,stemcells,cancercelllines,andMSCs—contacttech@systembio.comtolearnmore).

EXOCETisahighlyquantitativeassayandthestandardsincludedwitheachkithavebeencalibratedusingNanoSightanalysis,enablingcalculationofthenumberofexosomesinyoursamples.TheEXOCETKitcontainsallofthenecessaryassayreagentsandanoptical96-wellplatetoperform96reactions.

Fast—completein20minutesAntibody-free—quantitationisbasedontheactivityofAChE,anenzymeenrichedinmostexosomesQuantitative—includedcalibrationstandardsenablecalculationofthenumberofexosomeparticlesFlexIBLe—workswithallmammalianspeciestested(human,mouse,rat)Fullycompatible—workswithmostexosomeisolationmethodsincludingtheExoQuickfamilyofreagents,ultracentrifugation,immunoaffinitypurification,andchromatography

Choosetheexosomequantitationmethodthat’sbestforyourstudies

ExoELISA-ULTRACD63DetectionExoELISACD9
ExoELISACD63
ExoELISACD81EXOCETFluoroCetUseForfastandsensitiveantibody-basedquantitationofexosomesForsensitivequantitationofexosomeswhentimeandinputsamplearenotlimitingForfastquantitationofextracellularvesicleswithmoderatesampleinputrequirementsForthemostsensitivequantitationofextracellularvesicleswithverylowsampleinputrequirementsDetectionmethodAntibodyAntibodyEnzymaticEnzymaticQuantitationchemistryEnzymatic(HRP)Enzymatic(HRP)ColorimetricFluorescentTotalprotocoltime4hours(noovernightincubation)24hours20min60minInputsampleamount(proteinequivalent)1–200µg>500µg50µg

REFERENCES

SavinaA,etal.TheexosomepathwayinK562cellsisregulatedbyRab11.JCellSci.2002Jun15.115(Pt12):2505-15.PMID:12045221.GuptaS,andKnowltonAA.HSP60traffickinginadultcardiacmyocytes:roleoftheexosomalpathway.AmJPhysiolHeartCircPhysiol.2007Jun.292(6):H3052-6.PMID:17307989.

HowItWorks:

ExosomequantitationwithEXOCETisquickandeasy
Isolateexosomes(ExoQuick,ExoQuick-TC,ExoQuicKPLUS,andExoQuick-TCPLUSareallexcellentmethods)LyseexosomeswiththeincludedExosomeLysisBufferMeasureAChEactivity—addtheincludedbufferandincubatefor10–20minutes.Readoutisat405nm

Citations:Domenis,R,etal.(2017)CharacterizationoftheProinflammatoryProfileofSynovialFluid-DerivedExosomesofPatientswithOsteoarthritis.MediatorsInflamm..2017Jun21;2017:4814987.PMID:28634420Wu,X,etal.(2017)TongxinluoInhibitsRenalFibrosisinDiabeticNephropathy:InvolvementoftheSuppressionofIntercellularTransferofTGF-ββ1-ContainingExosomesfromGECSToGMCsReadMore:http://www.worldscientific.com/doi/abs/10.1142/S0192415X17500586.TheAmericanJournalofChineseMedicine.2017Jun29;.Link:TheAmericanJournalofChineseMedicineRody,WJ,etal.(2017)TheuseofcellcultureplatformstoidentifynovelMarkersofboneanddentinresorption.OrthodCraniofacRes.2017Jun1;:89-94.PMID:28643914Val,S,etal.(2017)PurificationandcharacterizationofmicroRNAswithinmiddleearfluidexosomes:implicationinotitismediapathophysiology.Pediatr.Res..2017Apr5;.PMID:28157838Slowey,PD.(2017)SalivaryDiagnosticsUsingPurifiedNucLeicacids.MethodsMol.Biol..2017Dec7;1537:3-15.PMID:27924585Sharma,R,etal.(2017)Detectionofphosphatidylserine-positiveexosomesforthediagnosisofearly-stagemalignancies.Br.J.Cancer.2017Jun22;.PMID:28641308Hurwitz,SN,etal.(2017)CD63RegulatesEpstein-BarrVirusLMP1ExosomalPackaging,EnhancementofVesicleProduction,andNoncanonicalNF-κBSignaling.J.Virol..2017Mar1;91(5).PMID:27974566Thomou,T,etal.(2017)ADIpose-derivedcirculatingmiRNAsregulategeneexpressioninothertissues.Nature.2017Feb23;542(7642):450-455.PMID:28199304Jiang,X,etal.(2017)Exosomesfrompediatricdilatedcardiomyopathypatientsmodulateapathologicalresponseincardiomyocytes.Am.J.Physiol.HeartCirc.Physiol..2017Apr1;312(4):H818-H826.PMID:28130338Hovsepian,T,Saint-Auret,G&Lebot,C.(2017)ASkinExtracellularSignalingPathwayviaIntercellularExosomesHighlightedbyGeneticandEpigeneticAnalysisAppliedtoaNaturalIngredient.29thIFSCCCongress.;:23-28.Link:29thIFSCCCongressColluru,VT.(2016)Anti-TumorDNAvaccination:OptimizingAntigenExpressionandPresentation.Thesis.;.Link:ThesisYang,J,etal.(2016)Anomalousuptakeandcirculatorycharacteristicsoftheplant-basedsmallRNAMIR2911.SciRep.2016Jun2;6:26834.PMID:27251858Lee,JY,etal.(2016)Circulatingexosomesfrompatientswithsystemiclupuserythematosusinduceanproinflammatoryimmuneresponse.ArthritisRes.Ther..2016Nov16;18(1):264.PMID:27852323Huynh,N,etal.(2016)CharacterizationofRegulatoryExtracellularVesiclesfromOsteoclasts.J.Dent.Res..2016Jun1;95(6):673-9.PMID:26908631Gutierrez,MJ,etal.(2016)AirwaySecretorymicroRNAomeChangesduringRhinovirusInfectioninEarlyChildhood.PLoSONE.2016Sep20;11(9):e0162244.PMID:27643599Rossaint,J,etal.(2016)Directedtransportofneutrophil-derivedextracellularvesiclesenablesplatelet-mediatedinnateimmuneresponse.NatCommun.2016Nov15;7:13464.PMID:27845343Matsumoto,Y,etal.(2016)Quantificationofplasmaexosomeisapotentialprognosticmarkerforesophagealsquamouscellcarcinoma.Oncol.Rep..2016Nov1;36(5):2535-2543.PMID:27599779Wu,XM,etal.(2016)Exosomesfromhighglucose-treatedglomerularendothelialcellsactivatemesangialcellstopromoterenalfibrosis.BiolOpen.2016Apr15;5(4):484-91.PMID:27010029Basappa,SN.(2015)EffectofcmvIL-10onExosomeProductionbyHumanBreastCancerCells.Thesis.;.Link:ThesisPeterson,MF,etal.(2015)Integratedsystemsforexosomeinvestigation.Methods.2015Oct1;87:31-45.PMID:25916618
SystemBiosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创蚂蚁淘技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。现阶段研发重心为RNA干扰（RNAi）研究之相关工具。SystemBiosciences(SBI)致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。SBIfocusesondevelopingunique,innovativetechnologiesthatprovideresearcherswiththetoolstoinvestigateandunderstandgenomicandproteomicfunction.Ourmissionistoprovidetoolsforthegenome-wideanalysisofthemechanismsthatregulatecellularprocessesandBIOLOGicalresponses.HeadquarteredinPaloAltoabout30milessouthofSanFrancisco,SBIisgeographicallysurroundedbyhighlysuccessfulresearchbiotechcompanies(e.g.,Affymetrix,LifeTechnologies,andGenentech)andsomeofthepremierlifescienceinstitutesintheworld,includingStanfordandtheUniversityofCalifornia,SanFrancisco.Asasmallprivatebiotechnologycompany,SBIcapitalizesonarichnetworkofconsultantsandcolleaguesandcontinuallycollaboratestoaccelerateitsdevelopmentofinnovativeandnovelapplications.Currently,SBIhaspartnershipswithoverthirtyscientistsfromseveralinstitutes.SystemBiosciences(SBI)consistsofahighlymotivatedteamcommittedtorealizingthepotentialofitsmissiontodevelopandbringtomarketuniqueandinnovativetechnologytoinvestigateandunderstandgenomicandproteomicfunction.Possessingadiverserangeofexperienceandknowledge,SBI"smanagementandstaffbringtheskills,talent,andinterestneededtosupportthecompany"scontinuingrapidgrowth.Astrongandprovenbackgroundincreativeresearchandproductdevelopmentwithproficiencyingeneticanalysis,microarraytechnology,andcellbiologyExtensivebusinessdevelopmentandmanagementexperienceAkeenunderstandingoftheneedsoflifescientistsExpertiseindeveloping,marketing,andsupportingconsistent,high-qualityresearchproductsAclearfocusontheneedtobuildandmaintainlastingcustomerrelationshipsthroughsupport,service,andclosecustomerinteraction

苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司，以常规生物学实验外包与生物学试剂为基础，放眼于生命科学领域的前沿技术服务，公司技术骨干有着多年的生物学科研背景，毕业于国内外名牌大学，并且有着丰富的项目经验，了解国内外科研行情，我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点，和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括：抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人，认真做事，服务至上“，希望用我们的专业知识和项目经验为国内科研客户解决科研问题，祝您科研之路畅通无阻！

SystemBiosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创蚂蚁淘技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。现阶段研发重心为RNA干扰（RNAi）研究之相关工具。
SystemBiosciences(SBI)致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。


SystemBiosciencescontinuestocreatenewanduniqueproductsaswellasimproveourwell-establishedproductlines.Ourcommitmenttoprovidingleading-edgetechnologiesismatchedbyourhighqualityofmanufacturingandqualitycontrolforallofourresearchreagentsandresearchprojectservices.