企业QQ
官方微信
| 货号： | HR100PA-1 |
| 供应商： | 上海觅拓 |
| 规格： | 10µg |
Getprecisegenomicintegrationofyourexpressioncassette
UsethePrecisionXTM BasicHRTargetingVectorforGeneKnock-In/Out(MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4)toinsertanyexpressioncassetteintoaspecificlocationofthegenomeforbothgeneknock-ins—i.e. toexpressagene-of-interestfromaspecificlocationsuchastheAAVS1SafeHarborSite—andgeneknock-outs—i.e.toknock-outagenebyinsertingspecificsequencessuchasaGFPreportercassette.
TheBasicHRTargetingVectorforGeneKnock-In/Out(MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4)featuresfourdifferentMCSs—cloneyourhomologyarmsintoMCS1andMCS4,yourexpressioncassetteintoMCS3,andanyadditionalsequencessuchassmallRNAsthatdon’tneedapolyAtailintoMCS2—aswellastwoLoxPsitesthatcanbeusedtoremovetheexpressioncassetteafteritisnolongerneeded(learnmoreaboutCre-LoxPexcisionhere).
WhyuseanHRtargetingvector?
Eventhoughgeneknock-outscanresultfromDSBscausedbyCas9alone,SBIrecommendstheuseofHRtargetingvectors(alsocalledHRdonorvectors)formoreefficientandprecisemutation.HRdonorscansupplyelementsforpositiveornegativeselectionensuringeasieridentificationofsuccessfulmutationevents.Inaddition,HRdonorscanincludeupto6-8kbofopenreadingframeforgeneknock-insortagging,and,whensmallmutationsareincludedineither5’or3’homologyarms,canmakespecific,targetedgeneedits.
ChoosetherightHRTargetingVectorforyourproject
| Catalog# | HRDonorVector | Features* | Application | |||
| GENE KNOCK-OUT | GENE KNOCK-IN | GENEEDITS | GENETAGGING | |||
| HR100PA-1 | MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4 | BasicHRDonor | √ | √ | ||
| HR110PA-1 | MCS1-EF1α-RFP-T2A-Puro-pA-MCS2 | RemovableRFPMarkerandpuromycinselection | √ | √ | ||
| HR120PA-1 | GFP-pA-LoxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCSPuro-pA-LoxP-MCS | TagwithGFPfusion RemovableRFPmarkerandpuromycinselection | √ | |||
| HR130PA-1 | T2A-GFP-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCSA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS | Co-expressGFPwith“tagged”geneviaT2A RemovableRFPmarkerandpuromycinselection | √ | |||
| HR150PA-1 | GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS | TagwithGFPfusionandco-expressluciferaseviaT2A RemovableRFPmarkerandpuromycinselection | √ | |||
| HR180PA-1 | IRES-GFP-pA-loxP-MCS1-EF1α-RFP-T2A-Puro-pA-LoxP-MCS2 | Co-expressGFPwith“tagged”geneviaIRES RemovableRFPmarkerandpuromycinselection | √ | |||
| HR210PA-1 | MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2 | RemovableGFPmarker,puromycinselection,andTKselection | √ | √ | ||
| HR220PA-1 | GFP-pA-LoxP-EF1α-RFP-T2A-Hygro-pA-LoxP-MCS | TagwithGFPfusion RemovableRFP,arkerandhygromycinSelection | √ | |||
| HR410PA-1 | MCS1-EF1α-GFP-T2A-Puro-pA-MCS2 | RemovableGFPmarkerandpuromycinselection | √ | √ | ||
| HR510PA-1 | MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2 | RemovableRFPmarkerandhygromycinselection | ||||
| HR700PA-1 | MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK | Enrichforon-targetintegrationwithnegativeTKselection** RemovableGFPmarkerandpuromycinselection | √ | √ | ||
| HR710PA-1 | MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2-PGK-hsvTK | Enrichforon-targetintegrationwithnegativeTKselection** RemovableRFPmarkerandhygromycinselection | √ | √ | ||
| HR720PA-1 | MCS1-EF1α-Blasticidin-pA-MCS2-PGK-hsvTK | Enrichforon-targetintegrationwithnegativeTKselection** Removableblasticidinselection | √ | √ | ||
| GE602A-1 | pAAVS1D-PGK-MCS-EF1α-copGFPpuro | FirstgenerationAAVS1-targetingHRDonor | √ | |||
| GE603A-1 | pAAVS1D-CMV-RFP-EF1α-copGFPpuro | FirstgenerationAAVS1-targetingHRDonor(positivecontrolforGE602A-1) | √ | |||
| GE620A-1 | AAVS1-SA-puro-MCS | SecondgenerationAAVS1-targetingHRDonor Promoterlesstoknock-inanygeneorpromoter-genecombination | √ | |||
| GE622A-1 | AAVS1-SA-puro-EF1α-MCS | SecondgenerationAAVS1-targetingHRDonor Constitutiveexpressionofyourgene-of-interest | √ | |||
| GE624A-1 | AAVS1-SA-puro-MCS-GFP | SecondgenerationAAVS1-targetingHRDonor Createreportercelllines | √ | |||
| CAS620A-1 | AAVS1-SA-puro-EF1α-hspCas9 | Knock-inCas9totheAAVS1site | ||||
| PBHR100A-1 | MCS1-5PBTR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3PBTR-MCS2 | UsewiththePiggyBacTransposonSystem Enablesseamlessgeneeditingwithnoresidualfootprint(i.e.completelyremovevectorsequences) | √ | |||
| *AllHRTargetVectorsexceptPBHR100A-1containLoxPsites.AnysequencesthatareintegratedbetweenthetwoLoxPsitescanberemovedthroughtransientexpressionofCreRecombinase. **ThecleverdesignoftheseHRDonorsenablesenrichmentforon-targetintegrationevents.APGK-hsvTKcassetteisincludedoutsideofthehomologyarms.Becauseofthisconfiguration,on-targetintegrationthatresultsfromhomologousrecombinationwillnotincludethePGK-hsvTKcassette—onlyrandomly-integratedoff-targeteventswillleadtointegrationofPGK-hsvTKandresultingTKactivity.Therefore,TKselectionwillnegativelyselectagainstoff-targetintegrants.Clickonanyoneofthesevectorstoseeadiagramofhowthenegativeselectionworks. | ||||||