AMSBIO Introduces New Reagents for Organoid Growth and Harvesting

AMSBIOIntroducesNewReagentsforOrganoidGrowthandHarvestingHits:1196Date:5/6/2016AMSBIOhasintroducedtwonewproductstoassisttheincreasingnumberofscientistsadoptingorganoidcultureasaphysiologicallyrelevantmodelfororgandevelopment.

ThesenewtoolsbuilduponthewidespreaduseofAMSBIO’sBME2‘organoidmatrix’byleADIngresearchgroupsworldwide.BME2istheextracellularmatrixofchoiceforgrowingandsupportingorganoidculturesthatareincreasinglybeingadoptedindrugscreeningapplications.

AnewR-Spondin1celllinehasbeendevelopedthatprovidesacost-effectivesolutionbywhichlabscanobtainalong-termsupplyofR-Spondin1(RSPO1),whichisacriticalingredientusedinthemaintenanceandproliferationofmouseandhumanorganoidProgenitorstemcells.R-SpondinspositivelyregulateWnt/beta-cateninsignaling,mostprobablybyactingasaligandforLGR4-6receptorsandaninhibitorforZNRF3.Our293TcelllineisstablytransfectedtoexpressmurineRSPO1withanN-terminalHAepitopetagandfusedtoaC-terminalmurineIgG2aFcfragmentAsaconsequencethiscelllinecanbeusedtoproduceeitherpurifiedRSPO1orRSPO1conditionedmedia.ThemurineRSPO1proteinfromthiscelllinehasbeendemonstratedtolowerthecostoforganoidcultureandtomaintainLgr5+stemcells.

AMSBIOhasalsointroducedaneworganoidharvestingsolutionthataddressesthechallengeofseparatingintactorganoidsfromextracellularproteinsinthehydrogelforpassaging,cryopreservation,orbiochemicalanalysis;andtofacilitatePDXxenograftmodels

Typicallyorganoidculturesexhibitcellularbehaviorsandmorphologiessimilartothoseseeninvivo.Unfortunately,adaptingthesemodelstostudybiochemicalprocesseshasbeenimpededbyproblemsassociatedwithseparatingintactorganoidsfromtheextracellularproteinsthatcomprisethehydrogel.Commonly,proteaseshavebeenemployedtodegradetheseextracellularproteins,butproteasetechniqueshavethedisadvantagesthattheyalsodegradeproteinsonthecellsurface,andthatproteaseactivitymaycarryoverintosubsequentculturesorlysatepreparations.TheCultrexOrganoidHarvestingSolutionfromAMSBIOprovidesanewnon-enzymaticmethodfordepolymerizingextracellularmatrixproteins,enablingharvestingofintactorganoidsfordownstreamapplications.

Forfurtherinformationonthesenewproductspleasevisithttp://www.amsbio.com/organoids.aspxorcontactAMSBIOon+44-1235-828200/+1-617-945-5033/info@amsbio.com.

Foundedin1987,AMSBiotechnology(AMSBIO)isrecognizedtodayasaleadingcompanycontributingtotheaccelerationofdiscoverythroughtheprovisionofcutting-edgelifesciencetechnology,productsandservicesforresearchanddevelopmentinthemedical,nutrition,cosmeticsandenergyindustries.AMSBIOisabletodrawuponin-depthexpertiseinextracellularmatricestoprovideelegantsolutionsforstudyingcellmotility,migration,invasionandproliferation.Widelyacknowledgedasanexpertincellculture,AMSBIOpartnerswithclientsintailoringcellsystemstoenhanceorganoidandspheroidtypescreeningoutcomesfromatechnologicalandcost-effectiveperspective.
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