The UCHT1 (Leu-4)(T3) monoclonal antibody reacts with human CD3ε a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3εis one of five polypeptide chains that combine to form the TCR complex. CD3εis expressed on T lymphocytes NK-T cells and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling T lymphocyte activation and antigen recognition. Crosslinking of the TCR via immobilized UCHT1 (Leu-4)(T3) antibody is commonly used to activate T cells in vitro.
Isotype | Mouse IgG1, κ |
Recommended Isotype Control(s) | InVivoMAb mouse IgG1 isotype control, unknown specificity(BE0083) |
Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
Immunogen | Human CD3ε |
Reported Applications |
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Endotoxin |
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Purity |
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Formulation |
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Sterility | 0.2 μM filtered |
Production | Purified from tissue culture supernatant in an animal free facility |
Purification | Protein G |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
RRID | AB_2687713 |
Molecular Weight | 150 kDa |
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