pIMAGO is a universal phosphoprotein detection technology, that is a reliable alternative to phosphoantibodies for Western blot or ELISA, and a safe and cost effective alternative to 32P radioactive assays. It enables sensitive and specific recognition of phosphorylated molecules.
Unlike phosphoantibodies, the binding is not biased by amino acid sequence, and therefore can be used for detection of any phosphorylation event on any protein site. The pIMAGO detection protocol resembles a simple Western blot procedure and is easy to incorporate into any laboratory.
pIMAGO is based on water soluble, globular nanopolymers (i.e., dendrimers) that are multifunctionalized with reactive groups (e.g., Ti(IV) ions) for the highly specific recognition of phosphate groups, and with “reporting” groups (e.g., biotin, fluorophore) that facilitate their detection. Multiple functionalized groups per dendrimer ensure very strong binding to the phosphoproteins (resulting in high specificity) and significant signal amplification from many “reporting” groups (resulting in high sensitivity).
Avidin–HRP Detection | Avidin–Fluor680 Detection |
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Amount of protein mix loaded (in ng) | Amount of protein mix loaded (in ng) |
JNK1–cJun Kinase Assay | Cdk1–Rb Kinase Assay |
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pIMAGO α-cJun Overlay | pIMAGO α-cJun Overlay |
Browse our full Western blotting range
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn